Biology of Reproduction

Department managerRychtářová Jana, Ing., Ph.D.
Parent departmentDeputy Director of Research
Description

EMBRYO BIOTECHNOLOGY

The aim is to use animal models for clarifying and addressing human reproduction issues and embryo technology methods for the preservation of endangered species.

We focus on developing new embryo technology and preservation of biological material from animals in the “genetic resource” category. The methods include namely the micro-handling of reproductive cells and embryos, derivation of stem cells, epigenetic characterisation of embryos and preservation of biological material using non-conventional methods. Methods are being developed through trials on animal models which could increase the efficiency of human assisted reproduction.

REGULATION AND MATURING OF MAMMALIAN OOCYTES

The aim is to produce high quality oocytes for the reproduction biotechnology purposes.

Research in the reproduction of farm animal females has two main directions: the first follows the direction of gaseous signalling molecules, so-called gas transmitters, on the maturing and fertilisation of mammalian oocytes; the second is concerned with the negative impacts of environmental pollutants on the mammalian oocyte quality. We pay special attention to agents capable of upsetting the hormonal balance of the organism, so-called endocrine disruptors.

The following groups are involved in the department:

Regulation and Maturing of Mammalian Oocytes
Embryo Biotechnology

Last publications:

TitleYear
PROKEŠOVÁ, Šárka, GHAIBOUR, Kamar, LIŠKA, František, KLEIN, Pavel, FENCLOVÁ, Tereza, ŠTIAVNICKÁ, Miriama, HOŠEK, Petr, ŽALMANOVÁ, Tereza, HOŠKOVÁ, Kristýna, ŘIMNÁČOVÁ, Hedvika, PETR, Jaroslav, KRÁLÍČKOVÁ, Milena and NEVORAL, Jan. Acute low-dose bisphenol S exposure affects mouse oocyte quality. Reproductive toxicology, 2020, 93, 19-27. ISSN 0890-6238. 2020
BENC, Michal, MARTÍNKOVÁ, Stanislava, RYCHTÁŘOVÁ, Jana, FULKA, Josef Jr., BARTKOVÁ, Alexandra, FULKA, Helena and LAURINČÍK, Jozef. Assessing the effect of interspecies oocyte nucleolar material dosage on embryonic development. Theriogenology, 2020, 155, 17-24. ISSN 0093-691X. 2020
RYCHTÁŘOVÁ, Jana, SZTANKÓOVÁ, Zuzana, HOFMANNOVÁ, Michala, VOSTRÝ, Luboš and MILERSKI, Michal. Characterization of the new genetic variant in the caprine lipoprotein lipase gene. Small Ruminant Research, 2020, 182, 5-10. ISSN 0921-4488. 2020
GAD, Ahmed, NĚMCOVÁ, Lucie, MURÍN, Matěj, KINTEROVÁ, Veronika, KAŇKA, Jiří, LAURINČÍK, Jozef, BENC, Michal, PENDOVSKI, Lazo and PROCHÁZKA, Radek. Global transcriptome analysis of porcine oocytes in correlation with follicle size. Molecular Reproduction and Development, 2020, 87, 102-114. ISSN 1040-452X. 2020
GELAUDE, Armance, SLABÝ, Sylvain, CAILLIAU, Katia, MARIN, Matthieu, LESCUYER.ROUSSEAU, Arlette, MOLINARO, Caroline, NEVORAL, Jan, KUČEROVÁ-CHRPOVÁ, Veronica, SEDMÍKOVÁ, Markéta, PETR, Jaroslav, MARTORIATI, Alain and BODART, Jean-Francois. Hydrogen Sulfide Impairs Meiosis Resumption in Xenopus laevis Oocytes. Cells, 2020, 9, Article Number 237. ISSN 2073-4409. 2020
ŘIMNÁČOVÁ, H., ŠTIAVNICKÁ, M., MORAVEC, J., CHEMEK, M., KOLINKO, Yaroslav, GARCÍA-ÁLVAREZ, Olga, MOUTON, P. R., TREJO, A. M. C., FENCLOVÁ, T., ERETOVÁ, N., HOŠEK, Petr, KLEIN, Pavel, KRÁLÍČKOVÁ, M., PETR, Jaroslav a NEVORAL, Jan. Low doses of Bisphenol S affect post-translational modifications of sperm proteins in male mice. Reproductive Biology and Endocrinology, 2020, 18, Article Number 56. ISSN 1477-7827. 2020
FONTANA, Josef, MARTÍNKOVÁ, Stanislava, PETR, Jaroslav, ŽALMANOVÁ, Tereza and TRNKA, Jan. Metabolic Cooperation in the Ovarian Follicle. Physiological Research, 2020, 69, 33-48. ISSN 0862-8408. 2020
FULKA, Helena, RYCHTÁŘOVÁ, Jana and LOI, Pasqualino. The nucleolus-like and precursor bodies of mammalian oocytes and embryos and their possible role in post-fertilization centromere remodelling. Biochemical Society Transactions, 2020, 48, 581-593, Part 2. ISSN 0300-5127. 2020
FULKA, Helena, OGURA, Atsuo, LOI, Pasqualino and FULKA, Jr. Josef. Dissecting the role of the germinal vesicle nuclear envelope and soluble. Journal of Reproduction and Development, 2019, 65, 433-441. ISSN 0916-8818. 2019
BENC, Michal, FULKA, Jr. Josef, STREJČEK, František, MOROVIČ, Martin, MURÍN, Matěj, MARTÍNKOVÁ, Stanislava, JETTMAROVÁ, Dominika and LAURINČÍK, Jozef. Enucleolation and nucleolus transfer in mammalian oocytes and zygotes. International Journal of Developmental Biology, 2019, 63, 253-258. ISSN 0214-6282. 2019
NEVORAL, Jan, LANDSMANN, Lukáš, ŠTIAVNICKÁ, Miriam, HOŠEK, Petr, MORAVEC, Jiří, PROKEŠOVÁ, Šárka, RIMNÁČOVÁ, Hedvika, KOUTNÁ, Eliška, KLEIN, Pavel, HOŠKOVÁ, Kristýna, ŽALMANOVÁ Tereza, FENCLOVÁ, Tereza, PETR, Jaroslav and KRÁLÍČKOVÁ, Milena. Epigenetic and non-epigenetic mode of SIRT1 action during oocyte meiosis progression. Journal of Animal Science and Biotechnology, 2019, 10, Article Nr. 67. ISSN 2049-1891. 2019
FULKA, Josef, Jr., BENC, Michal, LOI, Pasqualino, LANGEROVÁ, Alena and FULKA, Helena. Function of atypical mammalian oocyte/zygote nucleoli and its implications for reproductive biology and medicine. International Journal of Developmental Biology, 2019, 63, 105-112. ISSN 0214-6282. 2019
MURÍN, Matěj, STREJČEK, František, BARTKOVÁ, Alexandra, MOROVIC, Martin, BENC, Michal, PROCHÁZKA, Radek, LUCAS-HAHN, Andrea, PENDOVSKI, Lazo and LAURINČÍK, Jozef. Intranuclear characteristics of pig oocytes stained with brilliant cresyl blue and nucleologenesis of resulting embryos. Zygote, 2019, 27, 232-240. ISSN 0967-1994. 2019
VÝZKUMNÝ ÚSTAV ŽIVOČIŠNÉ VÝROBY, v.v.i. V UHŘÍNĚVSI. Culture medium eliminating maturation defects in mammalian oocytes caused by glyphosate. Authors: HOŠKOVÁ, Kristýna, ŽALMANOVÁ, Tereza, KOTT, Tomáš and PETR, Jaroslav.. Česká republika. Užitný vzor CZ 32522 U1. 2019-01-30. 2019
VÝZKUMNÝ ÚSTAV ŽIVOČIŠNÉ VÝROBY, v.v.i. V UHŘÍNĚVSI. Cultivation medium for eliminating maturation defects of mammalians induced by oocytes - nepetalakto. Authors: HOŠKOVÁ, Kristýna, ŽALMANOVÁ, Tereza, KOTT, Tomáš and PETR, Jaroslav.. Česká republika. Užitný vzor CZ 32480 U1. 2019-01-22. 2019
VÝZKUMNÝ ÚSTAV ŽIVOČIŠNÉ VÝROBY, v.v.i. V UHŘÍNĚVSI.. Culture medium eliminating maturation defects in mammalian oocytes caused by glyphosate. Authors: HOŠKOVÁ, Kristýna, ŽALMANOVÁ, Tereza, KOTT, Tomáš and PETR, Jaroslav.. Česká republika. Užitný vzor CZ 32520 U1. 2019-01-30. 2019
VÝZKUMNÝ ÚSTAV ŽIVOČIŠNÉ VÝROBY, v.v.i. V UHŘÍNĚVSI. Culture medium eliminating maturation defects in mammalian oocytes caused by glyphosate - demethoxyviridine. Authors: HOŠKOVÁ, Kristýna, ŽALMANOVÁ, Tereza, KOTT, Tomáš and PETR, Jaroslav.. Česká republika. Užitný vzor CZ 32521 U1. 2019-01-30. 2019
GAD, Ahmed, NĚMCOVÁ, Lucie, MURÍN, Matěj, KAŇKA, Jiří, LAURINČÍK, Jozef, BENC, Michal, PENDOVSKI, Lazo and PROCHÁZKA, Radek. microRNA expression profile in porcine oocytes with different developmental competence derived from large or small follicles. Molecular Reproduction and Development, 2019, 86, 426-439. ISSN 1040-452X. 2019
ŽALMANOVÁ, Tereza, HOŠKOVÁ, Kristýna, PROKEŠOVÁ, Šárka, NEVORAL, Jan and PETR, Jaroslav. Faster genetic progress by stallion substitutes. Náš chov, 2019, vol. 79(6), p. 56-57. ISSN . 2019
FULKA, Josef and FULKA, Helena. Nuclear and cytoplasmic transfer: Human applications and concerns.. In In Vitro Fertilization. Springer Nature Switzerland AG: Springer, 2019, s. 869-875. ISBN 978-3-319-43010-2. 2019
FULKA, Helena and LANGEROVÁ, Alena. Nucleoli in embryos: a central structural platform for embryonic chromatin remodeling?. Chromosome Research, 2019, 27, 129-140. ISSN 0967-3849. 2019
ŽALMANOVÁ, Tereza, HOŠKOVÁ, Kristýnaa, PROKEŠOVÁ, Šárka and PETR, Jaroslav. From Genome to Phenome. Náš chov, 2019, vol. 79(9), p. 21-24. ISSN 0027-8068. 2019
VÝZKUMNÝ ÚSTAV ŽIVOČIŠNÉ VÝROBY, v.v.i. V UHŘÍNĚVSI. Polymorphism detection kit (SNPs) in exon 7 of the beta casein gene (CSN2) in cattle. Authors: SZTANKÓOVÁ, Zuzana and RYCHTÁŘOVÁ, Jana.. Česká republika. Užitný vzor CZ 33426 U1. 2019-12-03. 2019
UNIVERZITA KARLOVA, UNIVERSITY OF MISSOURI, ČESKÁ ZEMĚDĚLSKÁ UNIVERZITA PRAHA a VÝZKUMNÝ ÚSTAV ŽIVOČIŠNÉ VÝROBY, v.v.i. V UHŘÍNĚVSI. Supplement for Cultivation of Mammalian Embryos. Authors: NEVORAL, Jan, YI, Young-Joo, ZÁMOSTNÁ-ADÁMKOVÁ, Kateřina, PETR, Jaroslav, SUTOVSKÝ, Peter and KRÁLÍČKOVÁ, Milena.. United States. United States Patent US 10,485,584 B2. 2019-11-26. 2019
BORKOVÁ, Markéta, SEYDLOVÁ, Růžena, DRAGOUNOVÁ, Hedvika, ELICH, Ondřej and RYCHTÁŘOVÁ, Jana. Presence of mastitis bacteria in a raw goat milk. Náš chov, 2019, vol. 79(4), p. 51-53. ISSN 0027-8068. 2019
VÝZKUMNÝ ÚSTAV ŽIVOČIŠNÉ VÝROBY, v.v.i. V UHŘÍNĚVSI. Utilization of polymorphis of genes lipogenic enzymes m to improve goat´s milk quality and its technological traits. Authors: SZTANKÓVA, Zuzana, RYCHTÁŘOVÁ, Jana, SVITÁKOVÁ, Alena and BORKOVÁ, Markéta.. Česká republika. Certifikovaná metodika 978-80-7403-216-5. 2019-09-30. 2019
VÝZKUMNÝ ÚSTAV ŽIVOČIŠNÉ VÝROBY, v.v.i. V UHŘÍNĚVSI. A method of eliminating glyphosate A-induced maturation defects in mammalian oocytes. Authors: HOŠKOVÁ, Kristýna, ŽALMANOVÁ, Tereza, KOTT, Tomáš and PETR, Jaroslav.. Česká republika. Patentový spis CZ 307943 B6. 2019-07-25. 2019
VÝZKUMNÝ ÚSTAV ŽIVOČIŠNÉ VÝROBY, v.v.i. V UHŘÍNĚVSI. A method of eliminating glyphosate B-induced maturation defects in mammalian oocytes. Authors: HOŠKOVÁ, Kristýna, ŽALMANOVÁ, Tereza, KOTT, Tomáš and PETR, Jaroslav.. Česká republika. Patentový spis CZ 307946 B6. 2019-07-25. 2019
VÝZKUMNÝ ÚSTAV ŽIVOČIŠNÉ VÝROBY, v.v.i. V UHŘÍNĚVSI. A method of eliminating glyph-induced C maturation defects in mammalian oocytes. Authors: HOŠKOVÁ, Kristýna, ŽALMANOVÁ, Tereza, KOTT, Tomáš and PETR, Jaroslav.. Česká republika. Patentový spis CZ 307949 B6. 2019-07-25. 2019
VÝZKUMNÝ ÚSTAV ŽIVOČIŠNÉ VÝROBY, v.v.i. V UHŘÍNĚVSI. A method of eliminating glyphosate C-induced maturation defects in mammalian oocytes. Authors: HOŠKOVÁ, Kristýna, ŽALMANOVÁ, Tereza, KOTT, Tomáš and PETR, Jaroslav.. Česká republika. Patentový spis CZ 307948 B6. 2019-07-25. 2019
LOI, Pasqualino, GALLI, Cesare, LAZZARI, Giovanna, MATSUKAWA, Kazutsugu, FULKA, Josef, Jr., GOERITZ, Frank and HILDEBRANDT, Thomas B. Development to term of sheep embryos reconstructed after inner cell mass/trophoblast exchange. Journal of Reproduction and Development, 2018, 64, 187-191. ISSN 0916-8818. 2018
NEVORAL, Jan, ŽALMANOVÁ, Tereza, HOŠKOVÁ, Kristýna, ŠTIAVNICKÁ, Miriam, HOŠEK, Petr, PETELÁK, Aleš and PETR, Jaroslav. Involvement of K-ATP(+) and Ca2+ channels in hydrogen sulfide-suppressed ageing of porcine oocytes. Biological Research, 2018, 51, pp.38. ISSN 0716-9760. 2018
NEVORAL, Jan, KOLÍNKO, Yaroslav, MORAVEC, Jiří, ŽALMANOVÁ, Tereza, HOŠKOVÁ, Kristýna, PROKEŠOVÁ, Šárka, KLEIN, Pavel, GHAIBOUR, Kamar, HOŠEK, Petr, ŠTIAVNICKÁ, Miriama, ŘIMNÁČOVÁ, Hedvika, TONAR, Zbyněk, PETR, Jaroslav and KRÁLÍČKOVÁ, Milena. Long-term exposure to very low doses of bisphenol S affects female reproduction. Reproduction, 2018, 156, 47-57. ISSN 1470-1626. 2018
MOROVIC, Martin, OSTRUP, Olga, STREJČEK, František, BENC, Michal, MURÍN, Matěj, JEDLIČKOVÁ, Katarína, BARTKOVÁ, Alexandra, LUCAS-HAHN, Andrea, PENDOVSKI, Lazo and LAURINČÍK, Jozef. Maternally inherited rRNA triggers de novo nucleolus formation in porcine embryos. Zygote, 2018, 26, 395-402. ISSN 0967-1994. 2018
HORVÁT, Filip, FULKA, Helena, JANKELE, Radek, MALÍK, Radek, JUN, Ma, ŠOLCOVÁ, Kateřina, SEDLÁČEK, Radislav, VLAHOVICEK, Kristian, SCHULTZ, Richard M. and SVOBODA, Petr. Role of Cnot6l in maternal mRNA turnover. Life Science Alliance, 2018, 1, 1-10. ISSN 2575-1077. 2018
NEVORAL, J., RÚČKA, V., KLEIN, Pavel, ŽALMANOVÁ, Tereza, HOŠKOVÁ, Kristýina, PROKEŠOVÁ, Š., ŠTIAVNICKÁ, M., PETR, Jaroslav and KRALICKOVA, M. The Interplay of Mouse and Porcine Biomodels for Endocrine Disruptor Reproductive Impairment. Slovak Journal of Animal Science, 2018, vol. 51(4), p. 161-170. ISSN 1337-9984. 2018
BENC, Michal, PENDOVSKI, Lazo, MURIN, Matěj, STREJČEK, František, MOROVIC, Martin, PROCHÁZKA, Radek and LAURINČÍK, Jozef. The oocyte´s nucleolus precursor body: The globe for life. Macedonian Veterinary Review, 2018, 41, 115-122. ISSN 1409-7621. 2018
ŽALMANOVÁ, Tereza, HOŠKOVÁ, Kristýna, NEVORAL, Jan, ADÁMKOVÁ, Kateřina, KOTT, Tomáš, ŠULC, Miloslav, KOTÍKOVÁ, Zora, PROKEŠOVÁ, Šárka, JÍLEK, František, KRÁLÍČKOVÁ, Milena and PETR, Jaroslav. Bisphenol S negatively affects the meotic maturation of pig oocytes  . Scientific Reports , 2017, 7, 485, 1-11. ISSN 2045-2322. 2017
LAURINČÍK, Jozef, STREJČEK, František, MOROVIČ, Martin, MURÍN, Matej, BENC, Michal, PENDOWSKI, Lazo a FULKA, Josef jr., 2017 Can we create a synthetic organism?. In Animal Physiology. Stará Lesná, Slovakia: Institute of Animal Physiology, s. 21. ISSN 2017
MOROVIC, Martin, STREJČEK, František, NAKAGAWA, Shoma, DESHMUKH, Rahul, S., MURIN, Matěj, BENC, Michal, FULKA, Helena, KYOGOKU, Hirohisa, PENDOVSKI, Lazo, FULKA, Josef, Jr. and LAURINČÍK, Josef. Mouse oocytes nucleoli rescue embryonic development of porcine enucleolated oocytes. Zygote, 2017, 25, 675-685. ISSN 0967-1994. 2017
BENC, Michal, STREJČEK, František, MURÍN, Matěj, MOROVIČ, Martin, MARTÍNKOVÁ, Stanislava, JETTMAROVÁ, Dominika, PENDOWSKI, Lazo, FULKA, Josef, jr .and LAURINČÍK, Jozef. Nucleologenesis and nucleolotransfer in mammalian oocytes: A review. Macedonian Veterinary Review, 2017, 40, 117-124. ISSN 1409-7621. 2017
ADÁMKOVÁ, Kateřina, YI, Young-Joo, PETR, Jaroslav, ŽALMANOVÁ, Tereza, HOŠKOVÁ, Kristýna, JELÍNKOVÁ, Pavla, MORAVEC, Jiří, KRÁLÍČKOVÁ, Milena, SUTOVSKÝ, Miriam, SUTOVSKÝ, Peter and SIRT1-dependent modulation of methylation and acetylation of histone H3 on lysine 9 (H3K9) in the zygotic pronuclei improves porcine embryo development. Journal of Animal Science and Biotechnology, 2017, 8, Article Nr. 83. ISSN 1674-9782. 2017
OOGA, Masatoshi, FULKA, Helena, HASHIMOTO, Satoshi, SUZUKI, Masatzaka G. and AOKI, Fugaku. Analysis of chromatin structure in mouse preimplantation embryos by fluorescent recovery after photobleaching. Epigenetics, 2016, 11, 85-94. ISSN 1559-2294. 2016
TŮMOVÁ, Lenka, CHMELÍKOVÁ, Eva, ŽALMANOVÁ, Tereza, KUČEROVÁ-CHRPOVÁ, Veronika, ROMAR., R, DVOŘÁKOVÁ, Markéta, HOŠKOVÁ, Kristýna and PETR, Jaroslav. Calcineurin role in porcine oocyte activation. animal, 2016, 10, 1998-2007. ISSN 1751-7311. 2016
NEVORAL, Jan, BODART, Jean-Francois and PETR, Jaroslav. Gasotransmitters in Gametogenesis and Early Development: Holy Trinity for Assisted Reproductive Technology-A Review. Oxidative Medicine and Cellular Longevity, 2016, 2016, e1730750. ISSN 1942-0994. 2016
FULKA, Helena and AOKI, Fugaku. Nucleolus precursor bodies and ribosome biogenesis in early mammalian embryos: old theories and new discoveries. Biology of Reproduction, 2016, 94, 143, 1-8. ISSN 0006-3363. 2016
DVOŘÁKOVÁ, M., HEROUTOVÁ, I., NĚMEČEK, D., ADÁMKOVÁ, K., KREJČOVÁ, T., NEVORAL, J., KUČEROVÁ CHRPOVÁ, V., PETR, Jaroslav and SEDMÍKOVÁ, M. The antioxidative properties of S-allyl cysteine not only influence somatic cells but also improve early embryo cleavage in pigs. PeerJ, 2016, 2016, e2280. ISSN 2167-8359. 2016

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