The objective of the current project is to facilitate suitability and development of Old Kladruber horses breed, especially in National Stud Kladruby nad Labem, by using most modern methods of research. Areas of serious problems to be solved are: – renewal and the quality improvement of grass vegetation on grassland belonging to National Stud Kladruby nad Labem, including the system of landscape maintenance – implementation of feed analysis to determine non-structural carbohydrates (NSC) and usage of its results to properly formulate feed rations for Old Kladruber horses. Implementation and application of effective nutrition programme in feeding system of Old Kladruber horses. – reduce occurrence frequency of orthopedic diseases, colic and other health disorders. – improving of the breeding system of the genetic resource Old Kladruber horse and introduction of the new method of reproduction. – determination of the appropriate size of population and the system of breeding and animal evaluation. – methods of preservation and usage of the frozen embryos and semen and modification of the studbook and genetic resources rules.