The objective of this study was to estimate the effect of a single-nucleotide polymorphism SNP11646 in the FGF2 (Fibroblast Growth Factor 2) gene on the deregressed proof of breeding values (DRP) of Holstein sires (n=149) for milk traits, i.e. milk yield, fat and protein yield and percentage. The effect of the polymorphism on the relative breeding value of own fertility, i.e. conception rate of heifers, cows and all females inseminated by the sperm of the sire, and the effect on relative breeding value for conception rate of daughters – heifers, cows and all females was evaluated as well. For AA sires, the average breeding values for milk production indicators were lower as compared to the other genotypes, but the differences were not significant. Contrarily, the same sires with AA genotype had the best breeding values for fertility both for maternal and direct genetic effect. For maternal effect, some differences were significant.