Genetic Variability of Lipogenic Enzymes (DGAT2, SCD) and Glycoprotein (BTN1A1) in the Dairy Goat Population of the Czech Republic
|Kateg. publikace||Příspěvky ve sbornících|
Enhancing milk productivity, yield and composition are of great economic importance for the dairy goat industry. They are influenced by multiple genes or quantitative loci (QTL) dispersed across the genome, and by an array of different environmental factors. The aim of this study was to investigate the variation/genetic polymorphism in the lipogenic enzymes DGAT2 and SCD and the glycoprotein BTN1A1 in the dairy goat population of the Czech Republic. Typing of the DGAT2, SCD and BTN1A1 loci was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Molecular analysis onfirmed the polymorphic site in exon 4 at position 216A > G and revealed that the most prevalent genotype was GG (76.09 percent) followed by AG (21.30 percent) and AA (2.61 percent) at the BTN1A1 locus in the studied goat population. The frequency of allele B (82.24 percent) and genotype BB (71.71 percent) predominated over allele A (14.47 percent) and genotypes AA (1.97 percent), AB (21.05 percent) and AC (3.95 percent), and allele C (3.29 percent) and genotype CC (1.32 percent) in the region of exon 3 and intron 3 at the SCD locus in the studied goat population. No polymorphism was detected in exon 4 at the DGAT2 locus. This information on genotype variability in the dairy goat population could be exploited in breeding programmes aiming to preserve biodiversity or improve the quality of processed milk from Czech goat breeds.
|Projekt||Vliv genetického polymorfizmu lipogenických enzymů na složení mléčného tuku a obsah mastných kyselin v mléce malých přežvýkavců (koze a ovce)|
|Oddělení||Genetika a šlechtění hospodářských zvířat|
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