Nuclear transfer of freeze-dried somatic cells into enucleated sheep oocytes

LOI, P., MATZUKAWA, K., PTAK, G., NATAN, Y., FULKA, Josef Jr. a ARAV, A. Nuclear transfer of freeze-dried somatic cells into enucleated sheep oocytes. Reproduction in Domestic Animals, 2008, 43, 417-422. ISSN 0936-6768.
Kateg. publikaceVědecké publikace impaktované
Interní odkaz8039.pdf
Abstrakt

In our experiments we have tested the viability of freeze-dried somatic cell nuclei after their injection into enucleated sheep oocytes. Several modifications of lyophilisation were developed. We have demonstrated that approximately 16% of reconstructedembryos with lyophilised somatic cell nuclei developed at least up to blastocyst stage.

OdděleníBiologie reprodukce
Pracovní skupinaEmbryobiotechnologie