SIRT1-dependent modulation of methylation and acetylation of histone H3 on lysine 9 (H3K9) in the zygotic pronuclei improves porcine embryo development

ADÁMKOVÁ, Kateřina, YI, Young-Joo, PETR, Jaroslav, ŽALMANOVÁ, Tereza, HOŠKOVÁ, Kristýna, JELÍNKOVÁ, Pavla, MORAVEC, Jiří, KRÁLÍČKOVÁ, Milena, SUTOVSKÝ, Miriam, SUTOVSKÝ, Peter a SIRT1-dependent modulation of methylation and acetylation of histone H3 on lysine 9 (H3K9) in the zygotic pronuclei improves porcine embryo development. Journal of Animal Science and Biotechnology, 2017, 8, Article Nr. 83. ISSN 1674-9782.
Kateg. publikaceVědecké publikace impaktované
Interní odkaz17196.pdf
Abstrakt

Background: The histone code is an established epigenetic regulator of early embryonic development in mammals. The lysine residue K9 of histone H3 (H3K9) is a prime target of SIRT1, a member of NAD+-dependent histone deacetylase family of enzymes targeting both histone and non-histone substrates. At present, little is known about SIRT1-modulation of H3K9 in zygotic pronuclei and its association with the success of preimplantation embryo development. Therefore, we evaluated the effect of SIRT1 activity on H3K9 methylation and acetylation in porcine zygotes and the significance of H3K9 modifications for early embryonic development. Results: Our results show that SIRT1 activators resveratrol and BML-278 increased H3K9 methylation and suppressed H3K9 acetylation in both the paternal and maternal pronucleus. Inversely, SIRT1 inhibitors nicotinamide and sirtinol suppressed methylation and increased acetylation of pronuclear H3K9. Evaluation of early embryonic development confirmed positive effect of selective SIRT1 activation on blastocyst formation rate (5.2 +/- 2.9% versus 32.9 +/- 8.1% in vehicle control and BML-278 group, respectively; P

ProjektInovace biotechnologií v reprodukci hospodářských zvířat, Rozvoj hospodářských zvířat v multifunkčním zemědělství
OdděleníBiologie reprodukce