Biology of Reproduction

Department managerRychtářová Jana, Ing., Ph.D.
Parent departmentDeputy Director of Research
Description

EMBRYO BIOTECHNOLOGY

The aim is to use animal models for clarifying and addressing human reproduction issues and embryo technology methods for the preservation of endangered species.

We focus on developing new embryo technology and preservation of biological material from animals in the “genetic resource” category. The methods include namely the micro-handling of reproductive cells and embryos, derivation of stem cells, epigenetic characterisation of embryos and preservation of biological material using non-conventional methods. Methods are being developed through trials on animal models which could increase the efficiency of human assisted reproduction.

REGULATION AND MATURING OF MAMMALIAN OOCYTES

The aim is to produce high quality oocytes for the reproduction biotechnology purposes.

Research in the reproduction of farm animal females has two main directions: the first follows the direction of gaseous signalling molecules, so-called gas transmitters, on the maturing and fertilisation of mammalian oocytes; the second is concerned with the negative impacts of environmental pollutants on the mammalian oocyte quality. We pay special attention to agents capable of upsetting the hormonal balance of the organism, so-called endocrine disruptors.

The following groups are involved in the department:

Regulation and Maturing of Mammalian Oocytes
Embryo Biotechnology

Last publications:

TitleYear
GIOIA, Luisa, PALAZZESE, Luca, CZERNIK, Marta, IUSO, Domenico, FULKA, Helena, FULKA, Josef Jr. and LOI, Pasqualino. Oocyte activation is a cytoplasm-confined event so far: what about the nucleus?. Reproduction, 2024, 167, Article number: 230360. ISSN 1470-1626. 2024
SZTANKÓOVÁ, Zuzana, MILERSKI, Michal, BRZÁKOVÁ, Michaela, RYCHTÁŘOVÁ, Jana a KYSELOVÁ, Jitka., 2023 Genetic diversity and population structure among Cen­tral European native sheep breeds using microsatellite markers. In 39th International Society for Animal Genetics. Cape Town, South Africa: ISAG, s. 25. ISSN 2023
SZTANKÓOVÁ, Zuzana, MILERSKI, Michal, BRZÁKOVÁ, Michaela, RYCHTÁŘOVÁ, Jana a KYSELOVÁ, Jitka., 2023 Genetic diversity and population structure among Central European native sheep breeds using microsatellite markers.. In 39th International Society for Animal Genetics. Cape Town, South Africa: ISAG, s. 167. ISSN 2023
MACHOVÁ, Karolína, HECTOR, Marina, ARRANZ, Juan Jose, PELAYO, Rocío, RYCHTÁŘOVÁ, Jana, MILERSKI, Michal, VOSTRÝ, Luboš and SUÁREZ-VEGA, Aroa. Genetic diversity of two native sheep breeds by genome-wide analysis of single nucleotide polymorphisms. animal, 2023, 17, Article number: 100690. ISSN 1751-7311. 2023
RYCHTÁŘOVÁ, Jana, KRUPOVÁ, Zuzana, BRZÁKOVÁ, Michaela, BORKOVÁ, Markéta, ELICH, Ondřej, DRAGOUNOVÁ, Hedvika, SEYDLOVÁ, Růžena and SZTANKÓOVÁ, Zuzana. Milk Quality, Somatic Cell Count, and Economics of Dairy Goats Farm in the Czech Republic. In Goat Science - Environment, Health and Economy. Dairying Public Utility Association, Hungary: Hungarian Sheep and Goat Dairying Public Utility Association, 2023, s. n. ISBN 978-1-78984-709-3. 2023
JÁNOŠÍKOVÁ, Martina, PETRIČÁKOVÁ, Kristýna, PTÁČEK, Martin, SAVVULIDI, Filipp Gregorijevic, RYCHTÁŘOVÁ, Jana and FULKA, Jr. Josef. New approaches for long-term conservation of rooster spermatozoa. Poultry Science, 2023, 102, Article number 102386. ISSN 1525-3171. 2023
FULKA, Helena, LOI, Pasqualino, CZERNIK, Marta, SURANI, Azim and FULKA, Jr. Josef. Omne vivum ex ovo: the oocyte reprogramming and remodeling activities. Reproduction, 2023, 165, R75-R89. ISSN 1470-1626. 2023
ŽALMANOVÁ, Tereza, HOŠKOVÁ, Kristýna, PROKEŠOVÁ, Šárka, NEVORAL, Jan, JEŠETA, Michal, BENC, Michal, YI, Young-Joo, MORAVEC, Jiří, MOČÁRYOVÁ, Beáta, MARTÍNKOVÁ, Stanislava, FONTANA, Josef, ELKALAF, Moustafa, TRNKA, Jan, ŽÁKOVÁ, Jana a PETR, Jaroslav. The bisphenol S contamination level observed in human follicular fluid affects the development of porcine oocytes. Frontiers in Cell and Developmental Biology, 2023, 11, Article number: 1145182. ISSN 2296-634X. 2023
PETR, Jaroslav. Anogenital distance as an early marker of dairy cattle fertility. Náš chov, 2022, vol. 82(2), p. 15. ISSN . 2022
ŘIMNÁČOVÁ, Hedvika, MORAVEC, Jiří, ŠTIAVNICKÁ, Miriama, HAVRÁNKOVÁ, Jiřina, MONSEF, Ladan, HOŠEK, Petr, PROKEŠOVÁ, Šárka, ŽALMANOVÁ, Tereza, FENCLOVÁ, Tereza, PETR, Jaroslav and NEVORAL, Jan. Evidence of endogenously produced hydrogen sulfide (H2S) and persulfidation in male reproduction. Scientific Reports, 2022, 12, Article number: 11426. ISSN 2045-2322. 2022
SZTANKÓOVÁ, Zuzana, RYCHTÁŘOVÁ, Jana, BRZÁKOVÁ, Michaela a MILERSKI, Michal., 2022 Genetic variability at the cluster of differentiation 14 (CD14) gene by PEA in dairy Czech goats. In ICG2022 13th International Conference on Goats. Online: International Goat Association, s. n. ISSN 2022
SZTANKÓOVÁ, Zuzana, RYCHTÁŘOVÁ, Jana, BRZÁKOVÁ, Michaela and SYRŮČEK, Jan. Goat breeding at the time of coronavirus. Náš chov, 2022, vol. 82(2), p. 33-35. ISSN 0027-8068. 2022
VACKOVÁ, Kristýna and ZAJÍČKOVÁ, Magdalena. In vitro production of cattle embryos. Náš chov, 2022, vol. 82(5), p. 20-22. ISSN . 2022
VACKOVÁ, Kristýna and RYCHTÁŘOVÁ, Jana. Intracytoplasmatic sperm injection and its use for conservation of genetic resources. Náš chov, 2022, vol. 82(9), p. 75-77. ISSN . 2022
VÝZKUMNÝ ÚSTAV ŽIVOČIŠNÉ VÝROBY, v.v.i. V UHŘÍNĚVSI. Culture medium for defects elimination of mammalian oocyte maturation induced by butylphenylmethylpropional - 2. Authors: PETR, Jaroslav, ŽALMANOVÁ, Tereza, PROKEŠOVÁ, Šárka, MOČÁRYOVÁ, Beáta and KOTT, Tomáš.. Česká republika. Užitný vzor CZ 36274 U1. 2022-08-16. 2022
VÝZKUMNÝ ÚSTAV ŽIVOČIŠNÉ VÝROBY, v.v.i. V UHŘÍNĚVSI. Culture medium for eliminating maturation defects of mammalian oocytes induced by butylphenylmethylpropional. Authors: PETR, Jaroslav, ŽALMANOVÁ, Tereza, PROKEŠOVÁ, Šárka, MOČÁRYOVÁ, Beáta and KOTT, Tomáš.. Česká republika. Užitný vzor CZ 36273 U1. 2022-08-16. 2022
ZAJÍČKOVÁ, Magdalena and VACKOVÁ, Kristýna. Calves born from in vitro produced embryos. Náš chov, 2022, vol. 82(9), p. 47-48. ISSN . 2022
FULKA, Helena, LOI Pasqualino, PALAZZESE, Luca, BENC, Michal and FULKA, Jr. Josef. Nucleus reprogramming/remodeling through selective enucleation (SE) of immature oocytes and zygotes: a nucleolus point of view. Journal of Reproduction and Development, 2022, 68, 165-172. ISSN 0916-8818. 2022
ZAJÍČKOVÁ, Magdalena and VACKOVÁ, Kristýna. In vitro Czech calves, Biotechnology in cattle breeding. Zpravodaj Českého svazu chovatelů masného skotu, 2022, vol. 2022(4), p. 44-45. ISSN . 2022
SZTANKÓOVÁ, Zuzana, BORKOVÁ, Markéta, RYCHTÁŘOVÁ, Jana, SMOLOVÁ, Jana, ELICH, Ondřej, ŠVEJCAROVÁ, Martina and BRZÁKOVÁ, Michaela. LPL gene polymorphism in East Frisian sheep and its influence on the quality of yogurt drink. Náš chov, 2022, vol. 82(7), p. 39-41. ISSN 0027-8068. 2022
VÝZKUMNÝ ÚSTAV ŽIVOČIŠNÉ VÝROBY, v.v.i. V UHŘÍNĚVSI. Set of multiplex microsatellite markers for the detection of genetic diversity, study of parentage and characterization genetic structures of breeds and sheep populations. Authors: RYCHTÁŘOVÁ, Jana and SZTANKÓOVÁ, Zuzana.. Česká republika. Užitný vzor CZ 36058 U1. 2022-05-26. 2022
VÝZKUMNÝ ÚSTAV ŽIVOČIŠNÉ VÝROBY, v.v.i. V UHŘÍNĚVSI. Set of primers for the determination of genetic structure, diversity and paternity in goats by microsatellite analysis (STR). Authors: SZTANKÓOVÁ, Zuzana and RYCHTÁŘOVÁ, Jana.. Česká republika. Užitný vzor CZ 36057 U1. 2022-04-26. 2022
VÝZKUMNÝ ÚSTAV ŽIVOČIŠNÉ VÝROBY, v.v.i. V UHŘÍNĚVSI. A single nucleotide polymorphism detection kit in the promoter of the bovine myogenic transcription factor MYF5 gene. Authors: KYSELOVÁ, Jitka, JEČMÍNKOVÁ, Kateřina, BARTOŇ, Luděk, SZTANKÓOVÁ, Zuzana and RYCHTÁŘOVÁ, Jana.. Česká republika. Patentový spis CZ 309115 B6. 2022-02-09. 2022
VACKOVÁ, Kristýna., 2022 Use of Interspecific ICSI for the Assessment of Buck Sperm DNA Damage After Different Methods of Cryopreservation. In Book of Abstracts CZU hybrid seminar Animal reproduction, sperm cryopreservation and analysis: an international experience. Prague: Czech University of Life Sciences, s. n. ISSN 2022
ŽALMANOVÁ, Tereza, HOŠKOVÁ, Kristýna, PROKEŠOVÁ, Šárka, MOČÁRYOVÁ, Beáta, KOTT, Tomáš and PETR, Jaroslav. Use of nanoparticles in livestock reproduction. Náš chov, 2022, vol. 82(2), p. 46-47. ISSN . 2022
VÝZKUMNÝ ÚSTAV ŽIVOČIŠNÉ VÝROBY, v.v.i. V UHŘÍNĚVSI. Goat polymorphism (SNPs) detection kit in exon 7 and 9 of the beta casein gene (CSN2) in goats. Authors: SZTANKÓOVÁ, Zuzana, RYCHTÁŘOVÁ, Jana and KYSELOVÁ, Jitka.. Česká republika. Užitný vzor CZ 34779 U1. 2021-01-19. 2021
BRZÁKOVÁ, Michaela, RYCHTÁŘOVÁ, Jana, ČÍTEK, Jindřich and SZTANKÓOVÁ, Zuzana. A Candidate Gene Association Study for Economically Important Traits in Czech Dairy Goat Breeds. Animals, 2021, 11, Article Number 1796. ISSN 2076-2615. 2021
MACHOVÁ, Karolína, MILERSKI, Michal, RYCHTÁŘOVÁ, Jana, HOFMANOVÁ, Barbora, VOSTRÁ VYDROVÁ, Hana, MORAVČÍKOVÁ, Nina, KASARDA, Radovan and VOSTRÝ, Luboš. Assessment of the genetic diversity of Two Czech autochthonous sheep breeds. Small Ruminant Research, 2021, 195, Article number 106301. ISSN 0921-4488. 2021
HOŠKOVÁ, Kristýna, ŽALMANOVÁ, Tereza, PROKEŠOVÁ, Šárka, MOČÁRYOVÁ, Beáta, KOTT, Tomáš and PETR, Jaroslav. Stress inheritance. Náš chov, 2021, vol. 81(11), p. 6-7. ISSN . 2021
NEVORAL, Jan, HAVRÁNKOVÁ, Jiřina, KOLINKO, Yaroslav, PROKEŠOVÁ, Šárka, FENCLOVÁ, Tereza, MONSEF, Ladan, ŽALMANOVÁ, Tereza, PETR, Jaroslav and KRÁLÍČKOVÁ, Milena. Exposure to alternative bisphenols BPS and BPF through breast milk: Noxious heritage effect during nursing associated with idiopathic infertility. Toxicology and Applied Pharmacology, 2021, 413, Article Number: 115409. ISSN 0041-008X. 2021
LOI, Pasqualino, CZERNIK, Marta, PALAZZESE, Luca, SCAPOLO, Pier Augusto, FULKA, Helena and FULKA, Josef Jr. Chapter 15 - Nuclear Transfer Technology and Its Use in Reproductive Medicine. In Manual of Intracytoplasmic Sperm Injection in Human Assisted Reproduction. Cambridge: Cambridge University Press, 2021, s. 148-153. ISBN 978-11-0888-759-5. 2021
BRZÁKOVÁ, Michaela, BOSKOVÁ, Iveta, VOSTRÝ, Luboš, RYCHTÁŘOVÁ, Jana and BUCEK, Pavel. Impact of COVID-19 on animal production in the Czech Republic. Animal Frontiers, 2021, 11, 47-50. ISSN  2160-6056. 2021
RYCHTÁŘOVÁ, Jana, LANGEROVÁ, Alena, FULKA, Helena, LOI, Pasqualino, BENC, Michal and FULKA, Josef Jr. Interspecific ICSI for the Assessment of Sperm DNA Damage: Technology Report. Animals, 2021, 11, Article Nr. 1250. ISSN 2076-2615. 2021
VÝZKUMNÝ ÚSTAV ŽIVOČIŠNÉ VÝROBY, v.v.i. V UHŘÍNĚVSI. Culture medium for eliminating bisphenol A or bisphenol S-1-induced maturation oocyte defects. Authors: ŽALMANOVÁ, Tereza, PROKEŠOVÁ, Šárka, MOČÁRYOVÁ, Beáta, KOTT, Tomáš and PETR, Jaroslav.. Česká republika. Užitný vzor CZ 35511 U1. 2021-11-04. 2021
VÝZKUMNÝ ÚSTAV ŽIVOČIŠNÉ VÝROBY, v.v.i. V UHŘÍNĚVSI. Culture medium for eliminating bisphenol A or bisphenol S-2-induced maturation oocyte defects. Authors: ŽALMANOVÁ, Tereza, PROKEŠOVÁ, Šárka, MOČÁRYOVÁ, Beáta, KOTT, Tomáš and PETR, Jaroslav.. Česká republika. Užitný vzor CZ 35512 U1. 2021-11-04. 2021
HOŠKOVÁ, Kristýna, ŽALMANOVÁ, Tereza, PROKEŠOVÁ, Šárka, MOČÁRYOVÁ, Beáta, KOTT, Tomáš and PETR, Jaroslav. RNA in cattle sperm. Náš chov, 2021, vol. 81(11), p. 20-21. ISSN . 2021
LOI, Pasqualino, PALAZZESE, Luca, SCAPOLO. Pier Augusto, FULKA, Jr. Josef, FULKA, Helena and CZERNIK, Marta. Scientific and technological approaches to improve SCNT efficiency in farm animals and pets. Reproduction, 2021, 162, F33-F43. ISSN 1470–1626. 2021
NĚMEČEK, David, CHMELÍKOVÁ, Eva, PETR, Jaroslav, KOTT, Tomáš and SEDMÍKOVÁ, Markéta. The effect of carbon monoxide on meiotic maturation of porcine oocytes. PeerJ, 2021, 9, Article number e10636. ISSN 2167-8359. 2021
SZTANKÓOVÁ, Zuzana, BORKOVÁ, M., RYCHTÁŘOVÁ, Jana, SMOLOVÁ, J., ELICH, O., ŠVEJCAROVÁ, M. and BRZÁKOVÁ, Michaela. The influence of lipoprotein lipase gene polymorphism in Czech East Friesian sheep on the fatty acid profile in milk and yoghurt drinks – a preliminary study. Journal of Animal and Feed Sciences, 2021, 30, 52-57. ISSN 1230-1388. 2021
PROKEŠOVÁ, Šárka, GHAIBOUR, Kamar, LIŠKA, František, KLEIN, Pavel, FENCLOVÁ, Tereza, ŠTIAVNICKÁ, Miriama, HOŠEK, Petr, ŽALMANOVÁ, Tereza, HOŠKOVÁ, Kristýna, ŘIMNÁČOVÁ, Hedvika, PETR, Jaroslav, KRÁLÍČKOVÁ, Milena and NEVORAL, Jan. Acute low-dose bisphenol S exposure affects mouse oocyte quality. Reproductive toxicology, 2020, 93, 19-27. ISSN 0890-6238. 2020
BENC, Michal, MARTÍNKOVÁ, Stanislava, RYCHTÁŘOVÁ, Jana, FULKA, Josef Jr., BARTKOVÁ, Alexandra, FULKA, Helena and LAURINČÍK, Jozef. Assessing the effect of interspecies oocyte nucleolar material dosage on embryonic development. Theriogenology, 2020, 155, 17-24. ISSN 0093-691X. 2020
HOŠKOVÁ, Kristýna, ŽALMANOVÁ, Tereza, PROKEŠOVÁ, Šárka, KOTT, Tomáš and PETR, Jaroslav. Cosmo bull: The promise of a sex shift in beef cattle. Náš chov, 2020, vol. 80(9), p. 54-56. ISSN . 2020
VOSTRÁ VYDROVÁ, Hana, HOFMANOVÁ, B., MORAVČÍKOVÁ, N., RYCHTÁŘOVÁ, Jana,KASARDA, Radovan, MÁCHOVÁ, K., BRZÁKOVÁ, Michaela and VOSTRÝ, Luboš. Genetic diversity, admixture and the effect of inbreeding on milk performance in two autochthonous goat breeds. Livestock Science, 2020, 240, Article number 104163. ISSN 1871-1413. 2020
MACHOVÁ, Karolína, HOFMANOVÁ, Barbora, RYCHTÁŘOVÁ, Jana, VOSTRÝ, Luboš, MORAVČÍKOVÁ, Nina and KASARDA, Radovan. Genetic variability analysis of 26 sheep breeds in the Czech Republic. Acta Fytotechnica et Zootechnica, 2020, 23, 38-45. ISSN 1336-9245. 2020
GAD, Ahmed, NĚMCOVÁ, Lucie, MURÍN, Matěj, KINTEROVÁ, Veronika, KAŇKA, Jiří, LAURINČÍK, Jozef, BENC, Michal, PENDOVSKI, Lazo and PROCHÁZKA, Radek. Global transcriptome analysis of porcine oocytes in correlation with follicle size. Molecular Reproduction and Development, 2020, 87, 102-114. ISSN 1040-452X. 2020
GELAUDE, Armance, SLABÝ, Sylvain, CAILLIAU, Katia, MARIN, Matthieu, LESCUYER.ROUSSEAU, Arlette, MOLINARO, Caroline, NEVORAL, Jan, KUČEROVÁ-CHRPOVÁ, Veronica, SEDMÍKOVÁ, Markéta, PETR, Jaroslav, MARTORIATI, Alain and BODART, Jean-Francois. Hydrogen Sulfide Impairs Meiosis Resumption in Xenopus laevis Oocytes. Cells, 2020, 9, Article Number 237. ISSN 2073-4409. 2020
RYCHTÁŘOVÁ, Jana, SZTANKÓOVÁ, Zuzana, HOFMANNOVÁ, Michala, VOSTRÝ, Luboš and MILERSKI, Michal. Characterization of the new genetic variant in the caprine lipoprotein lipase gene. Small Ruminant Research, 2020, 182, 5-10. ISSN 0921-4488. 2020
ŘIMNÁČOVÁ, H., ŠTIAVNICKÁ, M., MORAVEC, J., CHEMEK, M., KOLINKO, Yaroslav, GARCÍA-ÁLVAREZ, Olga, MOUTON, P. R., TREJO, A. M. C., FENCLOVÁ, T., ERETOVÁ, N., HOŠEK, Petr, KLEIN, Pavel, KRÁLÍČKOVÁ, M., PETR, Jaroslav a NEVORAL, Jan. Low doses of Bisphenol S affect post-translational modifications of sperm proteins in male mice. Reproductive Biology and Endocrinology, 2020, 18, Article Number 56. ISSN 1477-7827. 2020
FONTANA, Josef, MARTÍNKOVÁ, Stanislava, PETR, Jaroslav, ŽALMANOVÁ, Tereza and TRNKA, Jan. Metabolic Cooperation in the Ovarian Follicle. Physiological Research, 2020, 69, 33-48. ISSN 0862-8408. 2020
VOSTRÝ, Luboš, VOSTRÁ-VYDROVÁ, Hana, MORAVČÍKOVÁ, Nina, HOFMANOVÁ, Barbora, RYCHTÁŘOVÁ, Jana, MACHOVÁ, Karolína, BRZÁKOVÁ, Michaela and KASARDA, Radovan. Monitoring of genetic diversity in autochthonous Czech poultry breeds assessed by genealogical data. Czech Journal of Animal Science, 2020, 65, 224-231. ISSN 1212-1819. 2020
HOŠKOVÁ, Kristýna, ŽALMANOVÁ, Tereza, PROKEŠOVÁ, Šárka, KOTT, Tomáš and PETR, Jaroslav. Regression of two lost lines of Holstein cattle. Náš chov, 2020, vol. 80(8), p. 20-21. ISSN . 2020
HOŠKOVÁ, Kristýna, ŽALMANOVÁ, Tereza, PROKEŠOVÁ, Šárka and PETR, Jaroslav. Unexpected complication in DNA-edited cattle. Náš chov, 2020, vol. 80(6), p. 12-13. ISSN . 2020
VESELÁ, Zdeňka, BRZÁKOVÁ, Michaela, SVITÁKOVÁ, Alena and VOSTRÝ, Luboš. The first year of project: "Use of genomic information and optimization of beef cattle breeding programs". Zpravodaj Českého svazu chovatelů masného skotu, 2020, vol. 2020(1), p. 36-41. ISSN . 2020
FULKA, Helena, RYCHTÁŘOVÁ, Jana and LOI, Pasqualino. The nucleolus-like and precursor bodies of mammalian oocytes and embryos and their possible role in post-fertilization centromere remodelling. Biochemical Society Transactions, 2020, 48, 581-593, Part 2. ISSN 0300-5127. 2020

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