Nuclear and cytoplasmic determinants involved in the regulation of mammalian oocyte maturation and embryo development

Project no. 17-08605S
Category PROJEKTY GA ČR / Czech Science Foundation PROJECTS
ProviderGA ČR

Size is a fundamental biological feature that impacts physiology of the cell. This means not only the overal cell size but also the size of its organelles (subcellular structures). The basic aspect of size is its absolute value, having the upper and lower limits that are necessary for functional requirements. The mammalian oocyt or early-cleavage stage embryo represents, according to our opinion, an excellent experimental model systém. The present project aims to explore some fundamental questions of size scaling in mammalian oocytes and early embryos (nukleus, necleoli, chfomosomes, etc.). As some micromanipulation approaches are commonly used in understanding of nucleo-cytoplasmic interactions, size scalling mechanisms and realtedd consequences will enhance the efficiency of them.

Period2017 - 2019
Databse link (CZ)GA17-08605S

BENC, Michal, MARTÍNKOVÁ, Stanislava, RYCHTÁŘOVÁ, Jana, FULKA, Josef Jr., BARTKOVÁ, Alexandra, FULKA, Helena and LAURINČÍK, Jozef. Assessing the effect of interspecies oocyte nucleolar material dosage on embryonic development. Theriogenology, 2020, 155, 17-24. ISSN 0093-691X. 2020
FULKA, Helena, OGURA, Atsuo, LOI, Pasqualino and FULKA, Jr. Josef. Dissecting the role of the germinal vesicle nuclear envelope and soluble. Journal of Reproduction and Development, 2019, 65, 433-441. ISSN 0916-8818. 2019
FULKA, Josef, Jr., BENC, Michal, LOI, Pasqualino, LANGEROVÁ, Alena and FULKA, Helena. Function of atypical mammalian oocyte/zygote nucleoli and its implications for reproductive biology and medicine. International Journal of Developmental Biology, 2019, 63, 105-112. ISSN 0214-6282. 2019
FULKA, Helena and LANGEROVÁ, Alena. Nucleoli in embryos: a central structural platform for embryonic chromatin remodeling?. Chromosome Research, 2019, 27, 129-140. ISSN 0967-3849. 2019
BENC, Michal, PENDOVSKI, Lazo, MURIN, Matěj, STREJČEK, František, MOROVIC, Martin, PROCHÁZKA, Radek and LAURINČÍK, Jozef. The oocyte´s nucleolus precursor body: The globe for life. Macedonian Veterinary Review, 2018, 41, 115-122. ISSN 1409-7621. 2018
LAURINČÍK, Jozef, STREJČEK, František, MOROVIČ, Martin, MURÍN, Matej, BENC, Michal, PENDOWSKI, Lazo a FULKA, Josef jr., 2017 Can we create a synthetic organism?. In Animal Physiology. Stará Lesná, Slovakia: Institute of Animal Physiology, s. 21. ISSN 2017
MOROVIC, Martin, STREJČEK, František, NAKAGAWA, Shoma, DESHMUKH, Rahul, S., MURIN, Matěj, BENC, Michal, FULKA, Helena, KYOGOKU, Hirohisa, PENDOVSKI, Lazo, FULKA, Josef, Jr. and LAURINČÍK, Josef. Mouse oocytes nucleoli rescue embryonic development of porcine enucleolated oocytes. Zygote, 2017, 25, 675-685. ISSN 0967-1994. 2017
BENC, Michal, STREJČEK, František, MURÍN, Matěj, MOROVIČ, Martin, MARTÍNKOVÁ, Stanislava, JETTMAROVÁ, Dominika, PENDOWSKI, Lazo, FULKA, Josef, jr .and LAURINČÍK, Jozef. Nucleologenesis and nucleolotransfer in mammalian oocytes: A review. Macedonian Veterinary Review, 2017, 40, 117-124. ISSN 1409-7621. 2017