Characterization of the new genetic variant in the caprine lipoprotein lipase gene

RYCHTÁŘOVÁ, Jana, SZTANKÓOVÁ, Zuzana, HOFMANNOVÁ, Michala, VOSTRÝ, Luboš and MILERSKI, Michal. Characterization of the new genetic variant in the caprine lipoprotein lipase gene. Small Ruminant Research, 2020, 182, 5-10. ISSN 0921-4488.
Year2020
CathegoryScientific publication in impacted journals
Internal link20001.pdf
Abstract

Lipoprotein Lipase (LPL), a key enzyme in lipid metabolism, catalyses the hydrolysis of triglycerides (TG) from TG-rich lipoproteins, and serves a bridging function that enhances the cellular uptake of lipoproteins. By sequencing, we identified a four novel SNPs; one SNP involved a Gly→Arg AA substitution at position 23 of the signal peptide, and three SNPs in the intron I (KP261023) of the LPL locus. A protocol for rapid efficient simultaneous genotyping of LPL variants using the primer extension method (PEA) was described, and a total of 230 animals belonging to two goat breeds: White Shorthaired goat (n=177), and Brown Shorthaired goat (n=103) were genotyped. A total 15 genotype combinations were detected in tested goat populations.