Comparative study of the hypocholesterolemic and hypolipidemic activity of alginate and amidated alginate in rats
|Cathegory||Scientific publication in impacted journals|
Alginate is a copolymer of beta-D-mannuronate and alfa-L-guluronate, which are present in the cell wall of brown algae. The hypocholesterolemic and hypolipidemic activities of alginate and its derivative,which is prepared by a reaction with octadecylamine, were compared in rats fed diets containing cholesterol and palm fat at 10 and 50 g/kg, respectively. Amidated alginate at 20 g/kg significantly decreased serum cholesterol from 2.93 to 2.00 mikromol/mL, serum triacylglycerols from 1.66 to 0.92 mikromol/mL, hepatic cholesterol from 17.5 to 5.9 mikromol/g, and total hepatic lipids from 67.4 to 51.7 mg/g. Alginate at 20 g/kg significantly reduced hepatic cholesterol to 13.1 mikromol/g, but did not influence serum cholesterol, triacyl-glycerols, and total hepatic lipids. Amidated alginate significantly increased the faecal concentrations of neutral sterols from 98.7 to 122.4 mikromol/g DM, but decreased faecal concentration of bile acids from 19.4 to 14.0 mikromol/g DM. In samples of intestinal contents, taurine-conjugated bile acids dominated glycineconjugates. The supplementation of diets with cholesterol significantly increased the expression of hepatic cholesterol 7 alfa-hydroxylase, especially in rats that received cholesterol without alginate or amidatedalginate. In conclusion, amidated alginate is an effective hypocholesterolemic agent that is more efficient than its parent polysaccharide.
Volek Zdeněk, doc. Ing., Ph.D.
Skřivanová Eva, prof. MVDr., Ph.D.
Taubner Tomáš, Ing., Ph.D.
Dušková Dagmar, Ing.
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