Bacterial causes of bovine mastitis are usually ubiquitous gram-positive bacteria of the genera Streptococcus, Staphylococcus, Escherichia, etc. These bacteria present a risk of mammary gland infections in cattle, along with all its consequences. Their further danger is linked to humans, especially due to the very high degree of resistance of the bacteria to disinfectants and antibiotics. This resistance is then spread into the environment. It can be reduced in several ways, including the use of alternative disinfectants and antimicrobials. This study focuses on the use of oils rich in medium-chain fatty acids (MCFAs), which are known for their antibacterial activity and can serve as a substitute for antiseptics and antibiotics. The aim of the experimental part of this study is to evaluate by the in vitro microdilution broth method inhibitory activity of MCFA-rich palm oils after cleavage by exogenous lipase from Mucor javanicus against five strains of gram-positive bacterial pathogens that have been shown to cause mastitis in dairy cattle (Staphylococcus aureus and Streptococcus agalactiae). The hypothesis lies in the idea of using MCFA-rich palm oils as an alternative approach to „predip“ and „postdip“ practices on dairy cattle farms.