The objective of the study was to evaluate antimicrobial interactions between gentamicin (GEN), an antibiotic commonly used in veterinary medicine, and zinc pyrithion (ZnP) against Staphylococcus aureus strains. In vitro antimicrobial effect of GEN and ZnP was evaluated towards 7 strains of S. aureus by broth chequerboard method. Results show synergistic effect against all MSSA strains only at one concentration of tested substances. In the case MRSA, the combinatory effect of tested substances was not tested due to insolubility at high concentrations.