The panel of primers for the detection of the expression of markers that characterize equine mesenchymal stem cells

NOVÁKOVÁ, Z., VACKOVÁ, I., MOŠKO, T. & CZERNEKOVÁ, V. Panel primerů pro detekci exprese markerů charakterizujících mesenchymální kmenové buňky koně. 2013. Užitný vzor, CZ 26046 UI1, Výzkumný ústav živočišné výroby, v.v.i..{INTLINK}
VÝZKUMNÝ ÚSTAV ŽIVOČIŠNÉ VÝROBY, v.v.i. V UHŘÍNĚVSI. The panel of primers for the detection of the expression of markers that characterize equine mesenchymal stem cells. Authors: NOVÁKOVÁ, Zora, VACKOVÁ, Irena, MOŠKO, Tibor and CZERNEKOVÁ, Vladimíra.. Česká republika. Užitný vzor CZ 26046 UI1. 2013-11-04.
Year2013
CathegoryUtility patent, prototype, legal regulation, tecchnolgy
Internal link13144.pdf
Abstract

The invention relates to a new panel of primers for detecting the expression of markers characterizing mesenchymal stem cells of horse. Mesenchymal stem cells (MSC) are multipotent cells capable to repaire damaged mesenchymal and hematopoietic tissue. The study of MSC is currently on the rise due to their promising potential in regenerative medicine as the source material for tissue replacement and transplantation. The use of MSCs must precede their detailed characterization on cellular and molecular level. For this purpose a new panel of mRNA specific primers amplifying the selected genes characteristic for equine MSC was designed. This panel contains primers for the markers CD29, CD73, CD90, CD105, CD106, CD146, CD166 and TFPI-2. Using newly designed panel of specific primers refines the results of analysis and enables their correct interpretation, which is very important for further experimental work with MSCs.