The aim of this work was to compare whole white lupine seeds (WL, cv. Zulika) with a commonly used crude protein sources for rabbit’s diet. Two lactation diets were formulated. The first diet (SBM diet) contained soybean meal and sunflower meal as the main CP sources, whereas the second diet (WL diet) was based on WL. A total of 34 Hyplus rabbit does (17 animals per treatment) were fed 1 of the 2 lactation diets during the entire lactation (32 days). There was a higher daily milk production between d 2 and 21 (by 30 g; P=0.019) and for the entire lactation period (by 24 g; P=0.067) in rabbit does fed the WL diet than in does fed the SBM diet. The milk of does provided with the WL diet contained significantly less linoleic acid and more MUFA, a-linolenic acid and eicosapentaenic acid. A significantly lower solid feed intake and higher milk intake/solid feed intake ratio between d 17 and 32 of lactation of litters was observed in does fed the WL diet.