Differential gene expression associated with Corynebacterium pseudotuberculosis infection in sheep
Caseous lymphadenitis is a chronic disease caused by Corynebacterium pseudotuberculosis (C.pseudotuberculosis) which causes great economic waste in the small ruminants industry. In order to obtain detailed information about the pathogenesis and host immune response in naturally infected sheep, the gene expression difference was analyzed by high-throughput sequencing. An average of 26 796 transcripts were detected, representing 63% of all known sheep transcripts. Of the approximately 32 million sequences analyzed, 55-58% of reads were unambiguously mapped to the sheep reference genome. Resistant ewes from the affected herd had a total of 245 differentially expressed genes (DEG) compared to control, healthy females. A total of 195 DEG was evaluated in sick ewes. Cps infection has been the cause of increased regulation of 57 biological processes, mainly cell cycle, mitosis, meiosis, segregation of chromosomes and organelles, including control of these processes. Differentially expressed genes were classified into 54 different metabolic and signaling biochemical pathways that were affected by Cps infection and CLA disease. A total of 21 involved signaling pathways can be considered as controlling the immune response and maintaining homeostasis of a healthy organism.
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