The influence of lipoprotein lipase gene polymorphism in Czech East Friesian sheep on the fatty acid profile in milk and yoghurt drinks – a preliminary study
|Cathegory||Scientific publication in impacted journals|
The aim of the study was to evaluate the influence of single nucleotide polymorphism (SNP) in lipoprotein lipase gene (LPL) on the basic sheep milk parameters and the fatty acid profile in milk and yoghurt drinks made from it. The Primer Extension Analysis was used for genotyping 3 SNPs at positions: 74 (T/C), 130 (T/C), and 133 (T/A) in the 3´UTR region of the ovine LPL gene in 139 sheep of the East Friesian breed. According to the LPL genetic marker (CCCCAA and CTCTAT) 40 sheep were selected and divided into groups. Pooled milk was collected from May to June and was used to make yoghurt drinks with probiotics and prebiotics according to the genetic combination. In the pooled samples used for yoghurt production, the contents of fat, protein, lactose and total solids have been determined. The profile of fatty acid in fat from milk pooled samples as well as from yoghurt drinks has been analysed. Differences in the composition of milk and fatty acid profile were shown. The content of fat and total solids was significantly higher in the milk with the CTCTAT genotype combination in comparison to that with CCCCAA genotype. Moreover, these samples of milk and yoghurt products had a statistically lower content of palmitic acid and a significantly lower content of hypocholesterolemic acids. Targeted production of sheep milk from animals with different genotypes of the LPL may positively influence the fatty acid profile in milk and products made from it.
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