A simple method for assessment of hyaluronic acid production by cumulus-oocyte complexes
|Vědecké publikace impaktované
The cumulus expansion of cumulus-oocyte complex (COC) is an essential regulating process of oocyte maturation and as such is used as a biomarker of oocyte quality matured in vitro. Cumulus expansion is usually evaluated by non-invasive methods based on visual evaluation with many inaccuracies. However, determination of hyaluronic acid (HA), the most abundant compound of expanded cumuli, is one possible way of making a precise evaluation of cumulus expansion. Therefore, in this study the verification of HA analysis was performed, as an applicable approach to expanded cumuli evaluation and testing of oocyte maturation. The COCs were cultured in modified M199 medium for 8–48 h. The samples for HA analysis were prepared on an 8-hr time scale, and HA retained in COCs was measured using a spectrophotometric method adapted for this purpose. We observed an increasing quantity of HA during its in vitro cultivation. Comparison with expanded COCs‘ classification or expansion area reveals the proposed method of HA analysis as suitable for an evaluation of cumulus expansion in vitro. Our findings consider the quantity of HA-expressed cumulus expansion to be a valuable marker of COC quality and to provide an estimation of adequate oocyte meiotic stage.
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