Acute low-dose bisphenol S exposure affects mouse oocyte quality

PROKEŠOVÁ, Šárka, GHAIBOUR, Kamar, LIŠKA, František, KLEIN, Pavel, FENCLOVÁ, Tereza, ŠTIAVNICKÁ, Miriama, HOŠEK, Petr, ŽALMANOVÁ, Tereza, HOŠKOVÁ, Kristýna, ŘIMNÁČOVÁ, Hedvika, PETR, Jaroslav, KRÁLÍČKOVÁ, Milena a NEVORAL, Jan. Acute low-dose bisphenol S exposure affects mouse oocyte quality. Reproductive toxicology, 2020, 93, 19-27. ISSN 0890-6238.
Kateg. publikaceVědecké publikace impaktované
Interní odkaz20050.pdf

Bisphenol S (BPS) is widely used to replace the known endocrine disruptor BPA in various products. We evaluated the effect of acute in vivo BPS exposure on oocyte quality, simulating the oral route of exposure via oral gavage. Eight-week-old ICR female mice (N = 15 per experimental group) were exposed to vehicle or BPS1-BPS4 (0.001, 0.1, 10, and 100 ng BPS x g bw(-1) day(-1), respectively) for seven days. Oocytes were isolated and matured in vitro. We observed that BPS exposure increased aberrant spindle formation in mature oocytes and induced DNA damage. Moreover, BPS3 significantly increased the chromatin repressive marks 5-methyl cytosine (5meC) and H3K27me2 in immature oocytes. In the BPS2 group, the increase in 5meC occurred during oocyte maturation. Transcriptome analysis revealed differential expression of early embryonic development transcripts in BPS2-exposed oocytes. These findings indicate that the biological effect of BPS is non-monotonic, affecting oocyte quality even at concentrations that are orders of magnitude below those measured in humans.

ProjektDlouhodobý koncepční rozvoj výzkumné organizace
OdděleníBiologie reprodukce