The objective of this study was to evaluate main indicators of milk production on total 60 commercial dairy herds from the Czech Republic during a 9-yr period (2006-2014). Breakeven points and sensitivity analysis were used and associations of age at first calving (AFC), milk yield (MY) and calving interval (CI) were analyzed. Lowest AFC≤749 d showed the highest fertility, the lowest death rate of calves and lowest profitability without subsidies -2.49 %. Highest MY≥9,000 kg showed the highest fertility, lowest AFC, lowest feed costs and total costs 8.58 CZK (0.32 EUR) per L of milk and subsequent highest profitability 2.37 %. The analysis of fertility showed that herds with the lowest CI (≤389 d) achieved lowest cow depreciation costs 0.71 CZK (0.03 EUR) per L of milk, highest total costs 9.72 CZK (0.36 EUR) per L of milk and highest profitability 1.29 %. Breakeven points for the price of milk ranged between 7.81 and 8.75 CZK (0.29 and 0.32 EUR) per L in yr 2007 and 2014. Increase in input prices should adversely affect the increase the price of milk. The increase of price of milk by 1 % in 2014 will cause an increase in profit of CZK 745 (27.6 EUR) per cow per year.