The survival and effect of three new probiotic inoculants (Lactobacillus plantarum, L. fermentum, and Enterococcus faecium) on the nutritive value and fermentation parameters of corn silage was studied under laboratory conditions. It was detected that the microbial inoculants did not affect in vitro dry matter digestibility, as well as the concentration of total silage acids in inoculated corn silages. The three probiotic inoculants significantly or numerically lowered n-6:n-3 ratio of fatty acids in the inoculated silages and significantly decreased the concentration of C18:1 and significantly increased the concentrations of C18:2 and C18:3. The counts of inoculants decreased during ensiling of corn. In future, these inoculated corn silages will be used to study their effect on lipid metabolism in experiments in vitro (artificial rumen) and in vivo (cows).