Aberdeen Angus (AA), Charolais (CH), Simmental (SI), and Hereford (HE) bulls were used for determining breed differences in chemical composition, sensory characteristics, and fatty acid profile of MLLT. Intramuscular lipid contents were higher in AA andHE (P lt;0.05) than in CH and SI, but cholesterol contents were similar among the breed groups. The highest content of dry matter was found in HE (P lt;0.05), while AA had the lowest protein content (P lt;0.001). Meat from AA generally received the highest scores for different sensory characteristics (odour, flavour, texture, and juiciness). Concentrations of stearic acid in total muscle lipids were lower in SI than in CH (P lt;0.05), while total saturated fatty acids were lower in SI compared to CH (Plt;0.001) and AA (P lt;0.05). CH had less oleic acid and total monounsaturated fatty acids than AA (P lt;0.05), SI and HE (P lt;0.01). Concentrations of linolenic acid were highest in AA (P lt;0.01).