Cryopreservation of isolated mouse germinal vesicles

KŘEN, Radomír., FULKA,, Josef a FULKOVÁ, Helena. Cryopreservation of isolated mouse germinal vesicles. Journal of Reproduction and Development, 2005, 51, 289-292. ISSN 0916-8818.
Kateg. publikaceVědecké publikace impaktované
Interní odkaz5315.pdf

The storage of unfertilized oocytes, either immature, maturing or mature, is still unsatisfactory. Here we describe an approach in which germinal vesicles isolated as karyoplasts from immature oocytes are vitrified by the open pulled straws (OPS) methodin evacuated porcine zonae pellucidae. After thawing, their survival was almost absolute. Moreover, when thawed GV-karyoplasts were fused to immature oocyte cytoplasts the maturation of reconstructed cells resulted in the production of secondary oocytes-metaphase II.

OdděleníBiologie reprodukce
Pracovní skupinaEmbryobiotechnologie