The aim of the study was to evaluated the content of selected fatty acids in muscle tissue of pigs fed with feed mixtures with different sources of fat. Fourty crossbred gilts were divided into four groups and fed with control diet (C) or diet containing sunflower (S), linseed (L) or CLA. The highest content of linoleic acid was found in CLA and S group, the highest amount of alpha-linolenic acid was measured in L group. The content of arachidonic acid and EPA was also affected by the type of feeding mixture. The content of monounsaturated fatty acids (MUFA) was also significantly different in control and experimental groups. Similarly, the polyunsaturated fatty acids proportion was affected by the diet. The most favourable n-6/n-3 PUFA ratio was found in L group.