Effect of proteasome inhibitor MG132 on in vitro maturation of pig oocytes.

CHMELÍKOVÁ, Eva, SEDMÍKOVÁ, M., RAJMON, Radko, PETR, Jaroslav, ŠVESTKOVÁ, D. a JÍLEK, František. Effect of proteasome inhibitor MG132 on in vitro maturation of pig oocytes.. Zygote, 2004, 12, 157-162. ISSN 0967-1994.
Kateg. publikaceVědecké publikace impaktované
Interní odkaz4297.pdf
Abstrakt

The proteasome inhibitor MG132 blocked the exit of maturing pig oocytes from metaphase I stage. The blocking effect was experienced only when oocytes were exposed to an inhibitor before 18th hour ofin vitro culture. The eefects of MG132 are fully reversible. However, a significant portion of oocytes (46%) cultured for 48 hours in MG132-supplemented medium and then for 24 hour in MG132-free medium did not block meiosis at the stage of metaphase II and underwent spontaneous parthenogenetic activation.

OdděleníBiologie reprodukce