The aim of the study was to determine the effect of sunflower meal in feed mixture for fattening pigs on fatty acid composition of M. longissimus dorsi and backfat samples. Twenty crossbred gilts were divided into two groups and fed ad libitum with control diet (C) or diet containing sunflower meal (S). The content of saturated fatty acids (SFA) was higher in muscle tissue of pigs fed control diet but there was not found significant difference between control and experimental group in backfat samples. The monounsaturated fatty acids (MUFA) concentration was higher in both muscle and fat tissue of control pigs. On the other hand, total polyunsaturated fatty acids (PUFA) content was higher in muscle and fat samples of pigs fed sunflower diet. The n-6/n-3 PUFA ratio was lower in control group compared to experimental group.