The effect of various lupine seeds on milk production of rabbit does and performance of their litters before weaning was studied. Two isonitrogenous and isoenergetic lactation diets were formulated. The first lactation diet (WLS) contained white lupine seeds as the main crude protein source, whereas the second lactation diet (BLS) was based on blue lupine seeds. The WLS diet had higher ether extract content than the BLS diet. A total of 32 Hyplus does (16 per treatment, at the 3rd parturition) were fed one of these 2 diets. The litters were standardized to 9 kits on the day of birth. Feed intake, feed efficiency and milk production of does were not affected by dietary treatments, as well as performance of their litters. Live weight at weaning (P = 0.005), milk dry matter (P = 0.028) and fat contents (P = 0.016), as well as fat output per kg of metabolic weight (P < 0.001) were higher in does fed the WLS diet.