Effects of genistein and genistin on in vitro maturation of pig oocytes
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The effects of GEN (genistein ? an inhibitor of tyrosine protein kinase ? TPK) and GIN (genistin, an analogue of GEN without effects on TPK) on pig oocyte maturation and cumular cell expansion under in vitro conditions and connection with their estrogenic activity were tested. Further, the reversibility of GEN effects was verified. GEN blocked pig oocyte maturation at the stage of the germinal vesicle (GV), depending on the dose. After rinsing out the GEN the oocyte maturation recovered, but with abnormalities. As for GIN, with an increase in its concentration, the number of oocytes blocked at the GV stage significantly decreased, but the abnormal maturation increased. GEN inhibited the cumular cell expansion in proportion to its dose. GIN had a less pronounced effect. As GEN and GIN effects demonstrate similar patterns, it is probable that estrogenic activity is involved.
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