The objective of this study was to examine performance, carcass traits, muscle chemical composition, and blood and rumen metabolites of bulls fed diets with different levels of crude glycerine. A total of 48 Fleckvieh bulls were divided into four dietary treatment groups with different levels of glycerine supplementation. Feed intakes were recorded daily and the bulls were weighed every two weeks until slaughter. In addition, blood samples were collected. After slaughter, rumen fluid was collected, carcass characteristics were recorded, and m. longissimus lumborum composition was determined. No significant effect of glycerine inclusion was observed in any of the growth performance, carcass and meat quality traits studied. Also, no apparent effects on blood and rumen metabolites were detected. We conclude that crude glycerine can be used as a long-term substitution for barely meal up to the level of approximately 10 % of dry matter in the diets of finishing bulls.