There is a growing demand for selection on meat quality by Czech Aberdeen Angus breeders. The use of live animal ultrasound measures as indicator traits can be an efficient way to evaluate meat quality. The objective of this study was to estimate heritability and genetic correlations between live animal ultrasound measurements, such as rump fat thickness (P8FT), rib fat thickness (RBFT), eye muscle area (EMA), intramuscular fat content (IMF), and related traits, such as weight at scanning (SCW), and scrotal circumference (SC). The heritability estimates obtained in this study indicate that genetic evaluation for live animal ultrasound measures is feasible and genetic improvement through selection should be effective. However, the strong genetic correlation between intramuscular and subcutaneous fat is unfavorable and should be considered in the breeding program.