Hydrogen Sulﬁde Impairs Meiosis Resumption in Xenopus laevis Oocytes
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The role of hydrogen sulﬁde (H2S) is addressed in Xenopuslaevis oocytes. Three enzymes involvedinH2Smetabolism,cystathionineβ-synthase,cystathionineγ-lyase,and3-mercaptopyruvate sulfurtransferase, were detected in prophase I and metaphase II-arrested oocytes and drove an accelerationofoocytemeiosisresumptionwheninhibited. Moreover,meiosisresumptionisassociated with a signiﬁcant decrease in endogenous H2S. On another hand, a dose-dependent inhibition was obtained using the H2S donor, NaHS (1 and 5 mM). NaHS impaired translation. NaHS did not inducethedissociationofthecomponentsoftheM-phasepromotingfactor(MPF),cyclinBandCdk1, nor directly impacted the MPF activity. However, the M-phase entry induced by microinjection of metaphase II MPF-containing cytoplasm was diminished, suggesting upstream components of the MPF auto-ampliﬁcation loop were sensitive to H2S. Superoxide dismutase and catalase hindered the eﬀects of NaHS, and this sensitivity was partially dependent on the production of reactive oxygen species (ROS). In contrast to other species, no apoptosis was promoted. These results suggest a contribution of H2S signaling in the timing of amphibian oocytes meiosis resumption.
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