Book of abstracts comprises lectures presented at the international workshop (virtual) “Immunogenetics and breeding in livestock species” organized by the Department of Genetics and Breeding of the Institute of Animal Science (IAS) in Prague and held on November 8 – 9, 2022. The aim of the workshop was to update the current state in the immunogenetics of livestock species and to specify the ways of application of this knowledge to breeding programmes. The utilization of the variability of immune-related genes in breeding assumes different approaches than the formalized genomics, which is mostly based on the neutral SNPs. The high variability of immune genes, functional complexity and specificity of their products assumes the exploitation of related databases and informatic tools. The presentations at the workshop summed up the the bioinformatic resources and experimental approaches to the identification of causal genes, treated the examples of genetically determined infection resistance to the viral, bacterial and parasitic infections, and attempted to identify the cases of a high application potential in breeding. The organization of the workshop was related to the activities of the Genetics of Immune Response and Comparative MHC sections of the International Society for Animal Genetics (ISAG). The workshop was also considered to be related to the tradition of the immunogenetic research in the Czech Republic and to the previous workshop on this topic held in IAS on November 23 – 24, 2021. In total, 83 participants from 24 countries attended the workshop. The workshop was supported by the project LTV20020 of the INTER/VECTOR – INTER/EXCELLENCE programme of the EU.