The presentation focuses on the possibility of improvement of genomic evaluation via including of cows to the reference population. The project is defined with four consequent objectives, which are the optimization and automatization of DNA isolation from different biological samples, including the imputation of results from different sources to the unified format. SNP files will be used for Czech Holstein population studies (diversity, ROHs, inbreeding, etc.). Genotyped cows with own production will supplement the national reference population. Acquired results will be used for national genetic evaluation of Holstein, which is currently provided for milk production traits, longevity, type traits and fertility.