The study determined single nucleotide polymorphisms (SNPs) (rs68268260, rs68268250, rs55617172, rs68268263, rs68268268, rs43706433, rs68268256, rs68343167, rs43706434, rs68343168) in gene TLR2 encoding molecule Toll-like receptor 2. Collected data were used to analyze the effect of the individual TLR2 polymorphism on mastitis occurrence during the first lactation. GLM method was used for each SNP separately. The pilot model revealed the significant difference only for SNP rs43706433 (p=0.0311). Heterozygous dairy cows carrying genotype “TC” showed the significant difference in the occurrence of clinical mastitis at the first lactation.