The objective of this experiment was to determine susceptibility of Clostridium perfringens to C2-C18 fatty acids and to evaluate influence of pH in cultures grown on glucose. Lauric acid showed the highest antimicrobial activity among fatty acids tested. Its activity was not influenced by the presence of solid particles and did not cease at pH>6. Thus, lauric acid might be one of the means for control of clostridial infections in farm animals.