The aim of the study was to assess changes in quality of eggs from conventional, enriched cages and litter during the short-term (0, 1, 2, 3, 4 and 5 days) and long-term (0, 7, 14 and 21 days) storage at room temperature. During short-term storage significant interaction of time and housing was found mainly in albumen quality expressed by albumen height (P ≤ 0.012), Haugh units (P ≤ 0.029) and albumen pH (P ≤ 0.001). Haugh units decreased and albumen pH values increased faster in eggs from conventional cage, followed by eggs from litter and enriched cage. Egg weight more decreased within the 21-day storage in eggs from conventional cage (about 7.4%), while the smallest reduction of egg weight was in eggs from litter (about 5.0%). Minor changes occurred in Haugh units in eggs from enriched cage (reduction of 39.9). Interaction of long-term storage and housing system was revealed in Haugh units (P ≤ 0.001), albumen pH (P ≤ 0.026) and yolk index (P ≤ 0.006).