Grain legumes have the potential to become a sustainable alternative to other protein sources like soybean and rapeseed. Important representatives of the grain legumes used as livestock feed are certain species of lupines, which are characterised in terms of nutrient composition by their high protein and oil content. An experiment was conducted to examine the use of yellow and white lupine seed meals as a substitute for rapeseed in the diet of finishing bulls, and their effects on growth, carcass composition, and beef quality. Animals fed yellow lupine meal had lower live weight gains and inferior feed efficiency compared to the control rapeseed-fed group, while the results for white lupine were intermediary. All groups achieved comparable results for carcass composition and most meat quality traits. The proportions of fatty acids that are considered beneficial in terms of potential cardiovascular disease risk to consumers were lower in the meat of lupine-fed bulls.