The aim of our work was to create a complex screening system for assessing influences of prevention, prophylaxis and biosecurity practices on reducing usage of antimicrobial substances and AMR developing. We resulted from a professional literature (PubMed, Web of Science) especially the practical experience of the authors. The decision to use antimicrobials to treat humans and animals must be based on the veterinarian’s correct diagnosis, identifying the causative agent and therapy designing based on laboratory confirmation of antimicrobial susceptibility. Under no circumstances may antimicrobials in livestock farms be used to compensate for low hygiene, shortcomings in the work of management, zootechnics and caretakers.