Holstein cows were fed TMR supplemented with protected palm fat (PPF), rapeseed cake (RC) or extruded soyabean (ESB). PPF cows consumed significantly less feed and produced more milk than RC and ESB (32.9, 31.7 and 30.7 kg FCM.day-1, respectively). Milkfat from RC and ESB cows contained significantly more stearic, vaccenic and conjugated linoleic acid, and less palmitic acid then milk fat of PPF cows. The content of PUFA from ESB group was higher, the atherogenicity of its fatty acids was lower than inother cows, but the oxidative stability of milk fat was lower. RC significantly increased the spreadability index of butter. In ESB diet the crude protein was less degradable than in others. Body weights of RC and ESB increased by 11.13 and 15.6 kg, respectively, in PPF body weights decreased by 1.7 kg. Feeding oilseed-based supplements decreased milk yield and milk production efficiency somewhat, but improved the nutritional quality of milk fat.