The aim of this study was to evaluate semen quality together with the detection of sperm membrane lipid peroxidation in insemination doses of Přeštice black-pied boars during the season. Přeštice black-pied pigs are included in the program of preservation of Animal Genetic Resources in the Czech Republic. The basic parameters of semen quality in fresh boar semen were evaluated. Insemination doses (ID) were evaluated using flow cytometry and Computer Assisted Sperm Analysis (CASA). The quality of ID was divided according to the season. Flow cytometry was used to detect membrane lipid peroxidation (LPO). ID evaluated in the autumn were characterized by a significantly higher (P