Whole blood transcriptome analysis in sheep affected with caseous lymphadenitis.

KYSELOVÁ, Jitka, MARKOVÁ, J., SZTANKÓOVÁ, Zuzana, TICHÝ, Ladislav, MUŠKOVÁ, Michala, ŠLOSÁRKOVÁ, Soňa a BARTOŠOVÁ, B., 2021 Whole blood transcriptome analysis in sheep affected with caseous lymphadenitis.. In Abstract Book ISAG2021. www.isag.us: International Society for Animal Genetics, s. 89. ISSN
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Caseous lymphadenitis (CLA) is a chronic disease caused by the bacteria Corynebacterium pseudotuberculosis (Cps), which causes great economic losses in the small ruminant industry. In order to obtain detailed information about pathogen and host genome interaction in diseased and more resistant sheep, the gene expression was analyzed by the RNA-seq method. The blood samples were obtained both from Cps serologically positive (3) and negative ewes (3) considered resistant and from the health controls (3) of another herd. Trimmed reads were mapped to ovine transcriptome reference, and data were divided into three groups according to the health status and used for differential expression analysis employing the DESeq2. The transcripts with log fold change > 1,5 or < -1,5 and p < 0,05 were considered significantly differentially expressed. Genes were interpreted through the Gene Ontology system using the PANTHER classification. Gene Annotation and Pathway Mapping were provided using the KEGG database. Of the approximately 32 million sequences analyzed per sample, 55-58% of reads were unambiguously mapped to the reference. On average of 26 796 transcripts were detected, representing 63% of all known sheep transcripts. The transcripts were assigned to 12 295 genes. Resistant ewes from the affected herd had 245 differentially expressed genes (DEG), and diseased ones had 195 DEG compared to control females. Ewes infected with the Cps showed increased regulation of 57 biological processes, mainly cell cycle, and meiosis, segregation of chromosomes and organelles, including control of these processes. Differentially expressed genes were classified into 54 different metabolic and signaling biochemical pathways affected by Cps infection and CLA. Among them, up to 21 involved pathways can be considered to control the innate immune response. However, the intensive metabolism of both sick and more resistant ewes exposed to infectious pressure is evidenced by the fact that we did not find any significantly enriched biological processes or cellular structures in which there was lower gene expression than healthy individuals.

ProjektDlouhodobý koncepční rozvoj výzkumné organizace, Řešení problematiky výskytu bakteriálních, protozoárních a virových zoonotických agens v chovech malých přežvýkavců
OdděleníGenetika a šlechtění hospodářských zvířat