Nadřazené odděleníBiologie reprodukce
Karta skupiny karta_embryobiotechnologie_2017

Cílem je využívat zvířecí modely k objasnění a řešení humánních reprodukčních problémů.
Využití metod embryobiotechnologií pro záchranu ohrožených druhů zvířat.

Skupina se zaměřuje na vývoj nových embryobiotechnologií a uchování biologického materiálu od zvířat kategorie “Genetické zdroje”. Používané metody zahrnují zejména mikromanipulace zárodečných buněk a embryí, derivace kmenových buněk, epigenetickou charakterizaci vzniklých embryí a uchovávání biologického materiálu nekonvenčními postupy.
Zároveň jsou na základě pokusů na zvířecích modelech vyvíjeny metody, které by mohly zefektivnit postupy humánní asistované reprodukce – neinvazivní hodnocení vývojové potenciálu embrya, zvýšení aktivit regulujících opravu poškozené DNA v oocytech a zygotách, atd.

Obrázek 1: Klonované myši po přenosu jádra somatické buňky do enukleovaného oocytu.
Helena Fulková je jediná v ČR, která je schopna vyprodukovat SCNT klony myší.

Obrázek 2: Enukleolace oocytu myši.
Naše laboratoř jako první na světě vyvinula daný postup.

Fulka Josef, Ing., DrSc. vědecký pracovník
Fulková Helena, Mgr., Ph.D. vědecký pracovník
Rychtářová Jana, Ing., Ph.D. vědecký pracovník
Pačesová Petra technik

Poslední publikace:

Dissecting the role of the germinal vesicle nuclear envelope and soluble 2019
Enucleolation and nucleolus transfer in mammalian oocytes and zygotes 2019
Function of atypical mammalian oocyte/zygote nucleoli and its implications for reproductive biology and medicine 2019
Intranuclear characteristics of pig oocytes stained with brilliant cresyl blue and nucleologenesis of resulting embryos 2019
microRNA expression profile in porcine oocytes with different developmental competence derived from large or small follicles 2019
Nuclear and cytoplasmic transfer: Human applications and concerns. 2019
Nucleoli in embryos: a central structural platform for embryonic chromatin remodeling? 2019
Development to term of sheep embryos reconstructed after inner cell mass/trophoblast exchange 2018
Maternally inherited rRNA triggers de novo nucleolus formation in porcine embryos 2018
Role of Cnot6l in maternal mRNA turnover 2018
The oocyte´s nucleolus precursor body: The globe for life 2018
Mouse oocytes nucleoli rescue embryonic development of porcine enucleolated oocytes 2017
Nucleologenesis and nucleolotransfer in mammalian oocytes: A review 2017
Analysis of chromatin structure in mouse preimplantation embryos by fluorescent recovery after photobleaching 2016
Nucleolus precursor bodies and ribosome biogenesis in early mammalian embryos: old theories and new discoveries 2016
Can Nucleoli Be Markers of Developmental Potential in Human Zygotes? 2015
Absence of maternal cell contamination in mesenchymal stromal cell cultures derived from equine umbilical cord tissue 2014
Cell cycle regulation in cloning 2014
De novo formation of nucleoli in developing mouse embryos originating from enucleolated oocytes 2014
The maternal nucleolus plays a key role in centromere satellite maintenance during the oocyte to embryo transition 2014
Induced pluripotent stem (iPS) cells in domestic animals recent achievements - a review 2013
Interspecific somatic cell nuclear transfer: advancements and problems 2013
Klonování savců 2013
Nucleolar transplantation and human embryogenesis 2013
Pluripotent stem cells from maturing oocytes. 2013
Somatic cell nuclear transfer-derived embryonic stem cell lines in humans: pros and cons 2013
The ups and downs of somatic cell nucleus transfer (SCNT) in humans 2013
Dynamics of epigenetic remodeling in interspecies porcine zygotes 2012
Nuclear and cytoplasmic transfer: human applications and concerns 2012
Production of giant mouse oocyte nucleoli and assessment of their protein content 2012
RNA polymerase II transcriptional silencing in growing and fully grown germinal vesicle oocytes isolated from gonadotropin-stimulated and non-stimulated gilts 2012
Stem Cell Characteristics of Ovarian Granulosa Cells-Review 2012
Analysis of Marker Expression in Porcine Cell Lines Derived from Blastocysts Produced In Vitro and In Vivo 2011
Biological time machines: a realistic approach for cloning of extinct mammal 2011
Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species 2011
Genome of non-living cells: trash or recycle? 2011
Interspecies somatic cell nuclear transfer is dependent on compatible mitochondrial DNA and reprogramming factors 2011
Nucleoli from growing oocytes inhibit the maturation of enucleolated, full-grown oocytes in the pig 2011
Production of mouse embryonic stem cell lines from maturing oocytes by direct conversion of meiosis into mitosis 2011
Transplantation of nucleoli into human zygotes: not as simple as expected? 2011
Vitrification of fully grown and growing porcine oocytes using germinal vesicle transfer 2011
Development, embryonic genome activity and mitochondrial characteristics of bovine-pig inter-family nuclear transfer embryos 2010
Effect of antioestrogens on proliferation, differentiation and telomerase activity of pig granulosa cells in vitro 2010
Endocrine responses of eves to stress caused by different ways of milking 2010
Epigenetic Characteristics of Paternal Chromatin in Interspecies Zygotes 2010
Genome reprogramming during the first cell cycle in in vitro produced porcine embryos 2010
Nucleolar transplantation in oocytes and zygotes: challenges for further research 2010
Relationship between rank and plasma testosterone and cortisol in red deer males (Cervus elaphus) 2010
Effect of aromatase inhibitor (fadrozole) on proliferation, estradiol production and telomerase activity in pig granulosa cells in vitro 2009
How to repair the oocyte and zygote? 2009
Nucleoli in mammalian oocytes and embryos 2009
Použití prostředku na zjišťování genů v prasečích chromozomech 2009
The inability of fully grown germinal vesicle stage oocyte cytoplasm to transcriptionally silence transferred transcribing nuclei 2009
Asymmetric nuclear reprogramming in somatic cell nucfear transfer? 2008
Changes in global histone acetylation pattern in somatic celt nuclei after their transfer into oocytes at different stages of maturation 2008
Chromatin in early mammalian embryos: achieving the pluripotent state 2008
Cybrid human embryos-warranting opportunities to augment embryonic stem cell research 2008
Epigenetic analysis of human spermatozoa after their injection into ovulated mouse oocytes 2008
Freeze-dried somatic cells direct embryonic development after nuclear transfer 2008
Nuclear transfer of freeze-dried somatic cells into enucleated sheep oocytes 2008
Telomerase activity in pig granulosa cells proliferating and differentiating in vitro 2008
The maternal nucleolus is essential for early embryonic development in mammals 2008
Viability, acrosome morphology and fertilizing capacity of boar spermatozoa treated with strontium chloride 2008
Způsob detekce exprese prasečí telomerázy 2008
Degenerative action on mice and rat testes of polyspermine and its complexes with RNase A 2007
Molecular mechanisms of insulin-like growth factor 1 promoted synthesis and retention of hyaluronic acid in porcine oocyte-cumulus complexes 2007
Preweaning housing effects on behavior and physiological measures in pigs during the suckling and fattening periods 2007
Prostředek na zjišťování genů v prasečích chromozómech 2007
Putative embryonic stem cell lines from pig embryos 2007
Somatic cell nuclear transfer (SCNT) in mammals: The cytoplast and its reprogramming activities 2007
The use of micromanipulation methods as a tool to prevention of transmission of mutated mitochondrial DNA. 2007
Transmission of mitochondrial DNA disorders: Possibilities for the elimination of mutated mitochondria 2007
Development of pig embryos after electro-activation and in vitro fertilization in PZM-3 or PZM supplemented with fetal bovine serum 2006
DNA methylation pattern in pig in vivo produced embryos 2006
Immunohistochemical localization of proliferating cell nuclear antigen (PCNA) in the pig ovary 2006
No differences in the DNA methylation pattern in mouse zygotes produced in vivo, in vitro, or by intracytoplasmic sperm injection 2006
Placental abnormalities associated with post-natal mortality in sheep somatic cell clones 2006
The absence of a DNA replication checkpoint in porcine zygotes 2006
Cryopreservation of isolated mouse germinal vesicles 2005
Effect of protein supplement source on porcine oocyte maturation and subsequent embryonic development after parthenogenetic activation 2005
Germinal vesicle materials are requisite for male pronucleus formation but not for change in the activities of CDK1 and MAP kinase during maturation and fertilization of pig oocytes 2005
Mammalian oocyte therapies 2005
Developmental competence of bovine oocytes : Effect of follicle size and the phase of follicular wave on in vitro embryo production. 2004
Distribution of mitochondria in reconstructed mouse oocytes 2004
DNA methylation pattern in human zygotes and developing embryos 2004
Do cloned mammals skip a reprogramming step? 2004
Effect of caffeine on meiotic maturation of porcine oocytes 2004
Nucleolar dysfunction may be associated with infertility in humans 2004
Nucleus transfer in mammals: noninvasive approaches for the preparation of cytoplasts 2004
Oxytocin, prolactin and somatostatin in lactating sows: associations with mobilisation of body resources and maternal behaviour 2004
The role of adrenal androgens in antler growth of castrated fallow deer (Dama dama): Possible stimulation of adrenal secretion of testosterone by stress 2004