The aim of this study was to estimate genetic parameters for first calving interval in beef cattle population of the Czech Republic. Database of performance testing of 64 thousand of cows with repeated records was used to analyse. There were significant effects (P < 0.001) of age at first calving, herd-year-season, breed, calving difficulty and heterosis of cow (P = 0.038). Genetic parameters were estimated by AIREML. Heritability of first calving interval (0.1) was estimated.