The objective of this study was to assess the sperm membrane resistance in diluted boar semen with using short hypoosmotic swelling test (sHOS-test). sHOS-test evaluates the functional integrity of the spermatozoa plasma membrane, especially of the tail. sHOS-test involves incubation of spermatozoa in hypoosmotic solution 75 mOsm/l for 5 min. Eosin-nigrosine staining test was used with sHOS-test. Ejaculates from 21 fertile hybrid AI boars were collected by the gloved hand method. In the fresh boar semen were evaluated these parameters: semen volume, sperm motility, sperm concentration, morphologically abnormal spermatozoa, percentage of live sperm and sHOS-test. The boar semen was diluted in a semendilution rate of 1 + 4 in Androhep, Androstar, Androstar plus, MIII, LD and was stored at a temperature 17°C up to 96h. Samples of semen-dilution were kept at 38°C in water bath and resistance of spermatozoa was evaluated with sHOS-test before and after 1h incubation in water bath each day.