Common eland bulls are used for trophy hunting in South Africa with farming potential for meat. However, several assisted reproductive technologies (ARTs) can be applied to increase production and preserve their genetic materials, including the epididymal spermatozoa recovery after hunting or slaughtering. For this technique, collection period post-mortem is crucial for survivability and quality of spermatozoa for processing and cryopreservation. Hence, this study aimed to determine the effect of post-mortem collection time on the quality of spermatozoa recovered from the epididymis of common eland bulls. Six common eland bulls (2-2.5 years old;203±20 kilograms) were slaughtered.