A set of hybridization probes for determining the mutation in exon 7 of bovine beta-casein
Year | 2012 |
Cathegory | Utility patent, prototype, legal regulation, tecchnolgy |
Internal link | 12023.pdf |
Abstract | Technical solution regards determination of allelic variants A1, A2, A3 and B of milk protein β-casein in cattle by RT-PCR. Genotyping of allelic variants is performed with specific fluorescently labeled hybridization probes of the following composition: 1. For the detection of point mutations A 8101 C (NCBI GenBank M55158.1 USA) Sensor 8101 5 ‚CCA TCC GAC CTA ACA GCC TCC C-FL 3‘ Anchor 8101 5 ‚-CAA RED640 AAC ATC CCT CCT CTT ACT ACC CAA C-PH 3‘ 2. For the detection of point mutations C 8219 A (NCBI GenBank M55158.1 USA) Sensor 8219 5 ‚TCC GGC TAA GCA AAA AAT AgA-FL 3‘ Anchor 8219 5 ‚CCC-RED640 TTC AAA CCT CCA GTT TAT C-PH gag 3‘ 3. For the detection of point mutations C 8267 G (NCBI GenBank M55158.1 USA) Sensor 8267 5 ‚TAC TGA AAG CCA CCT GAC gag FL TC-3‘ Anchor 8267 5 ‚CAC TGA RED640-TGT AAA TGA CCT TCT GCA TCC-PH 3‘ Then the melting curve analysis of the hybrid probe – DNA fragment performed in the Light Cycler 480 II Real Time PCR system enabled subsequently the identification of the allelic variants A1, A2, A3 and B resulting in β-casein genotype determination. |
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