Method of determination allelic variants of kappa casein and beta-lactoglobulin in cattle

Výzkumný ústav živočišné výroby, v. v. i., Praha 10 - Uhříněves, CZ, . Způsob stanovení alelických variant kappa kaseinu a beta laktoglobulinu u skotu. Původce vynálezu: KYSELOVÁ, J., RYCHTÁŘOVÁ, J. & CZERNEKOVÁ, V. . Patentový spis 303083. (2012){INTLI
VÝZKUMNÝ ÚSTAV ŽIVOČIŠNÉ VÝROBY, v.v.i. V UHŘÍNĚVSI. Method of determination allelic variants of kappa casein and beta-lactoglobulin in cattle. Authors: KYSELOVÁ, Jitka, RYCHTÁŘOVÁ, Jana and CZERNEKOVÁ, Vladimíra.. Česká republika. Patentový spis CZ 303083 B6. 2012-02-13.
Year2012
CathegoryPatents
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Abstract

The solution relates to a determination of allelic variants of kappa casein and beta-lactoglobulin in cattle with touch down multiplex polymerase chain reaction (PCR). It is characterized by amplification of allelic variants performed with primers on the following composition: Kappa casein CSN3 F: 5 ‚- CCA TTG CTA GTG GTG CTA AGC C – 3 ‚(22 mer) and CSN3 R: 5‘ – CCT GCG TCT TCT TTG TTG ATG TC – 3 ‚(23 mer) with a length of 175 bp amplicon DNA and beta lactoglobulin LGB F: 5‘ – TTG TGC TGG ACA GCC ACT AC – 3 ‚(20 mer) LGB R: 5‘ – GCA GCG AGG ACC ACA CAG – 3 ‚(18 mer) with a length of 142 bp DNA amplicon. Then the melting curve analysis of specific heteroduplex DNA with high resolution (HRM) is performed in Light Cycler 480 II Real Time PCR system. HRM is newly applied and optimized for determination of genetic polymorphism of kappa casein and beta-lactoglobulin in cattle. The method determines the genotypes of kappa casein, variants A, B and E and beta-lactoglobulin, variants A and B accurately and simultaneously.