Enucleolation and nucleolus transfer in mammalian oocytes and zygotes

BENC, Michal, FULKA, Jr. Josef, STREJČEK, František, MOROVIČ, Martin, MURÍN, Matěj, MARTÍNKOVÁ, Stanislava, JETTMAROVÁ, Dominika and LAURINČÍK, Jozef. Enucleolation and nucleolus transfer in mammalian oocytes and zygotes. International Journal of Developmental Biology, 2019, 63, 253-258. ISSN 0214-6282.
CathegoryScientific publication in impacted journals
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The oocyte GV/GVs (germinal vesicle/germinal vesicles) and zygote PN/PNs (pronucleus/pronuclei) of some mammals contain clearly visible nucleoli which exhibit an atypical morphological structure. These nucleoli (NCLs) can be relatively easily manipulated, i.e. removed from GVs/PNs or eventually transferred into another oocyte/zygote. Thus, with the help of micromanipulation techniques it was possible to uncover the real function(s) they play in processes of oocyte maturation and early embryonic development. The purpose of our review is to describe briefly the micromanipulation techniques that can be used for oocyte/zygote nucleoli manipulation. Moreover, we present some examples of results that were obtained in nucleolus manipulation experiments.