Scientific and technological approaches to improve SCNT efficiency in farm animals and pets

LOI, Pasqualino, PALAZZESE, Luca, SCAPOLO. Pier Augusto, FULKA, Jr. Josef, FULKA, Helena and CZERNIK, Marta. Scientific and technological approaches to improve SCNT efficiency in farm animals and pets. Reproduction, 2021, 162, F33-F43. ISSN 1470–1626.
Year2021
CathegoryScientific publication in impacted journals
Internal link21053.pdf
Abstract

The birth of Dolly through somatic cell nuclear transfer (SCNT) was a major scientific breakthrough of the last century. Yet, while significant progress has been achieved across the technics required to reconstruct and in vitro culture nuclear transfer embryos, SCNT outcomes in terms of offspring production rates are still limited. Here, we provide a snapshot of the practical application of SCNT in farm animals and pets. Moreover, we suggest a path to improve SCNT through alternative strategies inspired by the physiological reprogramming in male and female gametes in preparation for the totipotency required after fertilization.